Naslov
Fluorescence spectral resolution of myelin basic protein conformers in complexes with lysophosphatidylcholine

Autori
Cavatorta, Paolo ; Masotti, Lanfranco ; Szabo, Arthur ; Juretić, Davor ; Riccio, Paolo ; Quagliariello, Ernesto

Izvornik
Cell Biophysics (1085-9195) 13 (1988), 3; 201-215

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
myelin basic protein ; fluorescence ; time-resolved fluorescence ; conformers ; protein-lipid interactions ; tryptophan fluorescence ; protein fluorescence

Sažetak
The structure of (Deibler) myelin basic protein in solution and in a lysolecithin lipid complex has been studied by using the emission properties of the single tryptophan residue of the protein (Trp-115). The studies have been carried out using both static and time-resolved fluorescence techniques. Relative to the free protein, the lipid bound myelin basic protein showed a, twofold increase in fluorescence intensity and a marked blue-shift in the emission maximum wavelength. The multiexponential fluorescence decays and the decay associated spectra indicated that the protein exists in at least three different conformations both in buffer and in lipids. Fluorescence polarization and acrylamide quenching experiments showed that the tryptophan containing region of the protein is embedded in the lipid matrix. The binding of the protein to the lipid appears to be comparable with that predicted for the interaction of amphipathic helices with nonpolar lipids.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Biologija

Napomena
Potrebno je ispraviti podatke o indeksiranosti
časposisa, jer je Cell Biophysics promijenio
naziv. Slijede podaci iz Wikipedia:
Cell Biochemistry and Biophysics is a peer-
reviewed scientific journal covering all aspects
of the biology of cells, especially their
biochemistry and biophysics. It was established
in 1979 as Cell Biophysics with Nicholas
Catsimpoolas as founding editor-in-chief,
obtaining its current name in 1996. The journal
is published by Springer Science+Business Media
and the editor-in-chief is Lawrence J. Berliner
(University of Denver).
Abstracting and indexing
The journal is abstracted and indexed in:

AGRICOLA
BIOSIS Previews[1]
Chemical Abstracts Service[2]
CSA Environmental Sciences
Elsevier Biobase
EMBASE[3]
EMBiology
Index Medicus/MEDLINE/PubMed[4]
Inspec[5]

Referativnyi Zhurnal
Science Citation Index Expanded[1]
Scopus[6]
According to the Journal Citation Reports, the
journal has a 2017 impact factor of 1.455.

POVEZANOST RADA