Naslov
Population structure of Cryphonectria hypovirus 1 across the invasion range of the chestnut blight fungus in central and south-eastern Europe

Autori
Ćurković Perica, Mirna ; Ježić, Marin ; Schwarz, Janine Melanie ; Prospero, Simone ; Sotirovski, Kiril ; Nuskern, Lucija ; Risteski, Mihajlo ; Krstin, Ljiljana ; Katanić, Zorana ; Poljak, Igor ; Idžojtić, Marilena ; Rigling, Daniel

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FEMS Online Conference on Microbiology - Electronic Abstract Book / - , 2020, 435-435

Skup
Federation of European Microbiological Societies Online Conference on Microbiology (FEMS 2020)

Mjesto i datum
Online, 28.10.2020. - 31.10.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biological control, population genetics, RNA virus

Sažetak
Cryphonectria parasitica is an introduced phytopathogenic fungus, that is causing chestnut blight, a devastating disease of chestnut trees. The disease can be controlled by a naturally occurring mycovirus Cryphonectria hypovirus 1 (CHV1). CHV1, which is a +ssRNA virus in the family Hypoviridae, reduces the virulence and fertility of C. parasitica, a phenomenon called hypovirulence which positively affects recovery of infected trees. Therefore, the prevalence of hypovirulent, CHV1-infected fungal strains in a population is correlated to efficacy of biological control of the disease. The aim of this study was to reveal prevalence and diversity of CHV1 in six C. parasitica populations, two of each in Switzerland, Croatia and North Macedonia. The prevalence of hypovirulent strains within populations ranged from 26% (Smolare, North Macedonia) to 75% (Contone, Switzerland). Ninety-five partial ORF- A CHV1 consensus sequences were obtained, ranging from 6 to 30 per population. Cluster analysis showed weak distinction between the obtained sequences placing them all within the Italian subtype of CHV1. The results suggest that all isolates analyzed in this study share a relatively recent common ancestor, probably spreading through Swiss and Croatan populations during the 2nd half of the 20th century and expanding to south-eastern Europe a couple of decades later. DAPC analysis distinguished North Macedonian from Swiss and Croatan CHV1 sequences, which was also clearly outlined by haplotype network. These results, as well as very close relationship amongst North Macedonian CHV1 strains, seem to support the hypothesis about the western European origin of south-east European CHV1 populations, with an observable bottleneck and founder effect.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Šumarstvo

POVEZANOST RADA