Naslov
Natural deep eutectic solvent as a unique solvent for valorisation of orange peel waste by the integrated biorefinery approach

Autori
Panić, Manuela ; Andlar, Martina ; Tišma, Marina ; Rezić, Tonči ; Šibalić, Darijo ; Cvjetko Bubalo, Marina ; Radojčić Redovniković, Ivana

Izvornik
Waste Management (0956-053X) 120 (2021); 340-350

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Biorefinery ; D-limonene ; Natural deep eutectic solvents ; Orange peel waste ; Pectin methylestrase ; Polyphenols ; (R)-1-phenylethanol ; SWOT analysis

Sažetak
This research investigates the use of seven natural deep eutectic solvents (NADESs) for valorisation of orange peel waste, with the final goal to propose a unique NADES for integrated biorefinery. Initial screening of NADESs revealed the excellent ability of cholinium-based NADES with ethylene glycol as hydrogen bond donor (ChEg50) to serve as a medium for orange peel-catalysed kinetic resolution (hydrolysis) of (R, S)-1-phenylethyl acetate with high enantioselectivity (ee = 83.2%, X = 35%), as well as it’s stabilizing effect on the hydrolytic enzymes (hydrolytic enzymes within ChEg50 peel extract were stabile during 20 days at 4 °C). The ChEg50 also showed a satisfactory capacity to extract D-limonene (0.5 mg gFW−1), and excellent capacity to extract polyphenols (45.7 mg gFW−1), and proteins (7.7 mg gFW−1) from the peel. Based on the obtained results, the integrated biorefinery of orange peel waste using ChEg50 in a multistep process was performed. Firstly, enantioselective kinetic resolution was performed (step I ; ee = 83.2%, X = 35%), followed by isolation of the product 1-phenylethanol (step II ; h = 82.2%) and extraction of polyphenols (step III ; h = 86.8%) from impoverished medium. Finally, the residual orange peel was analysed for sugar and lignin content, and results revealed the potential of waste peel for the anaerobic co-digestion process. The main bottlenecks and futures perspective of NADES-assisted integrated biorefinery of orange peel waste were outlined through SWOT analysis.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA