Naslov
Advanced glycation endproducts in human diabetic and non-diabetic cataractous lenses

Autori
Pokupec, Rajko ; Kalauz, Miro ; Turk, Nikša ; Turk, Zdenka

Izvornik
Graefes Archive for Clinical & Experimental Ophthalmology (0721-832X) 241 (2003), 5; 378-384

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Advanced glycation; Diabetes; Lens; Cataract

Sažetak
Background. Advanced glycation endproduct (AGE) formation is thought to contribute to aging and cataract formation in the lens. In this study, we evaluated AGE-immunoreactivity in human diabetic (n=14) and nondiabetic (n=31) cataractous lenses in relation to high-molecular-weight (HMW)-protein content, which is believed to contribute to the onset of cataract. Methods. AGE-immunoreactivity was detected in alkali-soluble individual lens samples. Competitive ELISA with polyclonal anti-AGE-antibody was performed to estimate AGEs. SDS-PAGE was used to detect changes in lens protein composition on the basis of molecular size. Results. Regression analysis of data from nondiabetic lenses showed a significant correlation between AGE lens content and patient age (r=0.665, p<0.001). The curve exhibited exponential regression (y=0.272&#61655; e0.025x). The level of nonspecified AGEs measured in diabetic lenses showed an overall increase as compared to nondiabetic lenses (4.03&#61617; 1.85 vs 1.78&#61617; 0.71 AU/mg protein, p<0.0078). SDS-PAGE showed the occurrence of HMW proteins in both diabetic and nondiabetic lens samples. However, in diabetic patients who had a higher level of AGEs, a significantly higher percentage of HMW proteins was also observed. The levels of AGE and percent of HMW aggregates showed a very significant correlation (r=0.68, p<0.007) in diabetic group, whereas in nondiabetics the correlation, although positive, did not reach statistical significance. Conclusion. The AGE distribution with a higher proportion in the samples of lenses rich in HMW aggregates corroborates the hypothesis that advanced glycation process might have a role in degenerative changes in eye lens, which in diabetic patients occur vigorously and much earlier than in those without diabetes.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA