Pregled bibliografske jedinice broj: 110095
Comet assay application in cell genome damage evaluation due to ionizing radiation in vitro
Comet assay application in cell genome damage evaluation due to ionizing radiation in vitro // Zbornik radova V. simpozija Hrvatskog društva za zaštitu od zračenja = Proceedings of the 5th Symposium of the Croatian Radiation Protection Association / Krajcar Bronić, Ines ; Miljanić, Saveta ; Obelić, Bogomil (ur.).
Zagreb: Hrvatsko društvo za zaštitu od zračenja, 2003. str. 156-161 (predavanje, međunarodna recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 110095 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Comet assay application in cell genome damage evaluation due to ionizing radiation in vitro
Autori
Želježić, Davor ; Garaj-Vrhovac, Vera
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Zbornik radova V. simpozija Hrvatskog društva za zaštitu od zračenja = Proceedings of the 5th Symposium of the Croatian Radiation Protection Association
/ Krajcar Bronić, Ines ; Miljanić, Saveta ; Obelić, Bogomil - Zagreb : Hrvatsko društvo za zaštitu od zračenja, 2003, 156-161
ISBN
953-96133-4-5
Skup
Simpozij Hrvatskog društva za zaštitu od zračenja (5 ; 2003)
Mjesto i datum
Stubičke Toplice, Hrvatska, 09.04.2003. - 11.04.2003
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
comet assay; gamma radiation; human lymphocytes; in vitro; dose-effect relationship
Sažetak
The aim of the present study was estimation of the possible application of comet assay in DNA damage evaluation caused by different gamma radiation doses in peripheral human lymphocytes in vitro. Therefor whole blood samples of young healthy, non-smoking donors were taken. The samples were divided in 4 specimens. The first specimen was used as the control. Other three specimens were irradiated using constant gamma irradiation source (60Co) giving the dose rate of 0.907 cGy/s. Different specimens were irradiated for 51 s, 437 s and 1099 s, giving the doses of 0.5 Gy, 4 Gy and 10 Gy. In order to estimate dose-response curve, on the control and all 3 irradiated whole blood samples comet assay under alkali conditions was performed. The comet assay endpoints showed higher values for all irradiated blood samples compared to the control. For both, tail length and tail moment, dose-effect relationship was found to be linear-quadratic in a dose range of 0.5Gy and 10 Gy. By this work wee also pointed out possible usage of the comet assay in the detection of DNA lesions caused by extremely high radiation dose, which is not possible by using standard cytogenetic methods.
Izvorni jezik
Engleski
Znanstvena područja
Biologija
POVEZANOST RADA
Projekti:
0022020
Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb
Profili:
Vera Garaj-Vrhovac
(autor)
