Naslov
Assessment of the systemic and local bone loss in the model of antigen induced arthritis.

Autori
Radanović, Igor ; Fadljević, Martina ; Lukač, Nina ; Katavić, Vedran ; Kovačić, Nataša.

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour - Abstract book / - , 2017, 63-63

Skup
2017 Annual Meeting of the Croatian Immunological Society with EFIS on Tour

Mjesto i datum
Zagreb, Hrvatska, 20.10.2017. - 21.10.2017

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
arthritis, systemic osteoresorption, local osteoresorption, inflammation

Sažetak
Introduction. Rheumatoid arthritis (RA) is a chronic autoimmune arthritis characterized by bone and cartilage destruction in the inflamed joints, as well as systemic osteopenia. Systemic and local bone loss involve separate mechanisms and have a different clinical significance. Among various murine models developed and applied in order to study pathogenic mechanisms of RA, antigeninduced arthritis (AIA) is considered to have few or no systemic effects. This model should exclusively mimic the local destructive changes. However, as the immunization protocol induces a systemic immune response we suspected that it may also affect bone mass at the systemic level. Aims. The objective of this study was to determine the extent of local bone destruction and generalized osteopenia in AIA. To achieve that, we assessed the trabecular bone volume in the second lumbar vertebrae (L2), femoral epiphyses and metaphyses, using a micro-computerized tomography. To assess the systemic effect of immunization, we assessed the proportion of myeloid (CD11b+Gr1+) cells in the spleen, femoral bone marrow and knee joints by flow cytometry. Materials and methods. 8-10 week old male C57BL/6 mice were divided into three groups: nonimmunized (NI), immunized control (CTRL), and arthritis group (AIA). Immunization protocol in both CTRL and AIA group consisted of two subcutaneous (s.c.) weekly injections of methylated bovine serum albumin (mBSA), followed by intraarticular (i.a.) injection of mBSA (AIA) or phosphatebuffered saline (PBS) (CTRL) two weeks after second injection. NI group was injected with PBS only at all points. Mice were sacrificed 10 days post i.a. injection, when L2, knee joints and spleens were harvested for analysis. Results. Statistically significant decreases in bone volume were found in the L2 and metaphyses of CTRL and AIA mice, in comparison to NI mice (p<0.05, Kruskal-Wallis test). On the other hand, the epiphyseal trabecular bone volume was significantly lower in AIA mice in comparison to ctrl and NI mice (p<0.05, Kruskal-Wallis test). In both immunized groups there was a significant increase in the proportion of myeloid cells in spleen, bone marrow and knee joints, in comparison to the control group. Conclusion. The AIA model of arthritis induces systemic osteopenia, which should be taken into account, in particular when addressing the local osteodestructive changes. Metaphyseal bone volume better reflects the systemic features of the model, whereas epiphyseal trabecular bone volume is a more reliable indicator of the local ostedestructive changes.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA