Naslov
MiR-7 AND miR-34a sequence cloning and expression in a1235 glioblastoma cell line

Autori
Kolić, Dora ; Horvat, Luka ; Šetinc, Maja ; Antica, Mariastefania ; Matulić, Maja

Izvornik
Molecular and experimental biology in medicine (2584-671X) 3 (2020), 2; 31-38

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
miR - 7 ; miR - 34a ; expression ; glioblastoma cell line

Sažetak
MiRNAs are small non-coding RNAs which have an important role in signalling circuits regulating different cell processes. miR-7 and miR-34a are known as tumour suppressors, and both of them can interfere with cell proliferation, differentiation, apoptosis and migration. We constructed plasmids containing pri-miRNA sequences for these two miRNAs and introduced them into the A1235 glioblastoma cell line. Clones containing increased expression of processed miR-7 and miR-34a were obtained. The proliferation and sensitivity to alkylation agent of transfected cells were similar to those of control cells. Our results indicate that an increase in miR-7 and miR34 expression alone in A1235 glioblastoma cells is not sufficient to change their proliferation or sensitivity to the influence of alkylating agents.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA