Naslov
Expression of KIT gene in testicular seminoma

Autori
Raos, Dora ; Mašić, Silvija ; Katušić Bojanac, Ana ; Bulić Jakuš, Floriana ; Ježek, Davor ; Ulamec, Monika ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Rad Hrvatske akademije znanosti i umjetnosti. Medicinske znanosti, 537 (2019), 46-47 / - , 2018, 74-74

Skup
Nikola Škreb symposium: New platforms in developmental biology - towards the clinical application

Mjesto i datum
Zagreb, Hrvatska, 29.11.2018. - 30.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
kit- gene ; testicular seminoma

Sažetak
Testicular neoplasia represents only 1% of all neoplasia, but it affects young male population. Approximately 95 % of all testicular cancers are germ cell tumors. There are two groups of TGCT, seminoma and non-seminoma. Compared to non– seminoma, seminoma is hypomethylated. The hypomethylated genome is unstable and more exposed to mutations what brings to the emergence of tumor cells. Hypomethylated genes show higher expression rate than other genes. Consequently, the gene expression pattern between tumor and healthy tissue differs. Gene expression on the protein level is used for diagnosis of tumors. OCT 3⁄4 is a gene which is already used as a specific marker for the diagnosis of seminoma, but new markers could contribute to diagnostic protocols and patient management. Archive seminoma slices were collected. Immunohistochemical staining was done manually, on 5μm archive tissue sections in the Laboratory for Epigenetics and Molecular Medicine. To visualize our genes of interest, we used specific antibodies. Morphometric analysis of gene expression at the protein level was performed at University Hospital Sestre Milosrdnice Department of Clinical Pathology Ljudevit Jurak. Differential gene expression in tumor tissue and in healthy tissue was observed. Analyzing and quantifying expression of genes at the protein level can contribute to the development of new histopathological markers for seminoma in blood and semen.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA