Naslov
Components comparison between human and experimental mouse teratocarcinoma
(Components comparison between human and experimental mouse teratocarcinoma)

Autori
Škara, Lucija ; Ulamec, Monika ; Mašić, Silvija ; Krasić, Jure ; Vujnović, Nebojša ; Katušić Bojanac, Ana ; Bulić-Jakuš, Floriana ; Ježek, Davor ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
27th Ljudevit Jurak International symposium on comparative pathology with one health session : final program & book of abstracts / - , 2019, 45-45

Skup
27th Ljudevit Jurak International symposium on comparative pathology with one health session

Mjesto i datum
Zagreb, Hrvatska, 31.05.2019. - 01.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
human ; mouse ; teratocarcinoma

Sažetak
Testicular germ cell tumors are the most frequent malignancies among young male population. Teratocarcinoma (TCa) is a type of TGCT composed of teratoma, which consists of tissues derived from all the three germ layers, and embryonal carcinoma. This type of tumor can be obtained experimentally by transplanting 7.5 days old mouse egg cylinder beneath the kidney capsule. Although this experimental tumor histologically resembles human teratocarcinoma, detailed comparison of human and mouse experimental TCa have not been performed yet and therefore it is the aim of our study. Twenty experimental mouse and eighteen human TCa formalin-fixed paraffin-embedded tissues were stained with hemalaun and eosin and their components were determined. Statistical analysis was performed by the Fisher's exact test. Mouse experimental TCa is, in average, composed of more different components (6.95) than human TCa (4.33). Statistically significant difference was found in presence of cells of all four tissue types. Stratified squamous and columnar epithelium, skeletal muscle, adipose tissue, glia and neural tube were statistically significant more frequent in mouse. Our results suggest that experimentally produced TCa diverge from human TCa in tissue types they are constituted of. Further analysis of proliferative and apoptotic activity, pluripotency and various cell type differentiation markers with immunohistochemical method and capillary western blot are in progress.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA