Naslov
Istraživanje preferencije vezanja proteina HB6, RDM1 i DMS3 na BPM1
(Preferential protein-protein binding of HB6, RDM1 and DMS3 to BPM1)

Autori
Tokić, Mirta

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Zagreb

Datum
15.02

Godina
2019

Stranica
68

Mentor
Bauer, Nataša

Neposredni voditelj
Markulin, Lucija

Ključne riječi
interakcija proteina ; kopurifikacija ; RdDM ; degradacija proteina ; BPM1 ; Arabidopsis thaliana
(protein interaction ; pull-down ; RdDM ; protein degradation ; BPM1 ; Arabidopsis thaliana)

Sažetak
BPM proteins in A. thaliana are known to direct several transcription factors to ubiquitinmediated 26S proteasome degradation. Preliminary investigation, after protein copurification by TAP-tag from Arabidopsis seedlings, has shown that BPM1 also potentially interacts with members of the RNA-directed DNA methylation (RdDM) pathway, DMS3 and RDM1. Here, interactions of BPM1 with transcription factor HB6 and proteins DMS3 and RDM1 were analyzed in one to one and one to two pull-down assays. For this purpose, suitable expression plasmids were generated using ligation or In- Fusion cloning methods. RosettaTM E. coli strain was chemically transformed with generated constructs. Protein expression was induced, and fusion proteins were purified. Finally, pull-down assays were performed and proteins visualized by SDS-PAGE, staining and immunodetection. In the conditions carried out here, RDM1 and DMS3 interacted with BPM1. Since HB6 interacted with both BPM1-GST and GST alone (negative control), a direct HB6 interaction could not be shown. These experiments provide a basis for future analyses which could elucidate the potential function of BPM1 in the RdDM pathway.

Izvorni jezik
Engleski

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