Pregled bibliografske jedinice broj: 1095844
The Serum and Saliva Proteome of Dogs with Diabetes Mellitus
The Serum and Saliva Proteome of Dogs with Diabetes Mellitus // Animals, 10 (2020), 12; 2261, 16 doi:10.3390/ani10122261 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1095844 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
The Serum and Saliva Proteome of Dogs with
Diabetes Mellitus
Autori
Franco-Martínez, Lorena ; Gelemanović, Andrea ; Horvatić, Anita ; Contreras-Aguilar, María Dolores ; Mrljak, Vladimir ; Cerón, José Joaquín ; Martínez-Subiela, Silvia ; Tvarijonaviciute, Asta
Izvornik
Animals (2076-2615) 10
(2020), 12;
2261, 16
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
canine diabetes mellitus ; gel-free proteomics ; high-resolution mass spectrometry ; tandem mass tags ; type 1 diabetes
Sažetak
This study aims to evaluate the changes in salivary and serum proteomes that occur in canine diabetes mellitus type-1 (DM) through a high-throughput quantitative proteomic analysis. The proteomes of 10 paired serum and saliva samples from healthy controls (HC group, n = 5) and dogs with untreated DM (DM group, n = 5) were analyzed using Tandem Mass Tags (TMT)-based proteomic approach. Additionally, 24 serum samples from healthy controls and untreated DM were used to validate haptoglobin in serum. The TMT analysis quantified 767 and 389 proteins in saliva and serum, respectively. Of those, 16 unique proteins in serum and 26 in saliva were differently represented between DM and HC groups. The verification of haptoglobin in serum was in concordance with the proteomic data. Our results pointed out changes in both saliva and serum proteomes that reflect different physiopathological changes in dogs with DM. Although some of the proteins identified here, such as malate dehydrogenase or glyceraldehyde-3-phosphate dehydrogenase, were previously related with DM in dogs, most of the proteins modulated in serum and saliva are described in canine DM for the first time and could be a source of potential biomarkers of the disease. Additionally, the molecular function, biological process, pathways and protein class of the differential proteins were revealed, which could improve the understanding of the disease’s pathological mechanisms.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti, Veterinarska medicina
POVEZANOST RADA
Projekti:
EK-FP7-621394 - Upgrading the research performance in molecular medicine at the Faculty of Veterinary Medicine University of Zagreb (VETMEDZG) (EK - FP7-ERAChairs-PilotCall-2013) ( CroRIS)
Ustanove:
Veterinarski fakultet, Zagreb,
Prehrambeno-biotehnološki fakultet, Zagreb,
Mediteranski institut za istraživanje života
Citiraj ovu publikaciju:
Časopis indeksira:
- Current Contents Connect (CCC)
- Web of Science Core Collection (WoSCC)
- Science Citation Index Expanded (SCI-EXP)
- SCI-EXP, SSCI i/ili A&HCI
- Emerging Sources Citation Index (ESCI)
- Scopus
