Naslov
Electrochemical adhesion based differentiation of algal cell species

Autori
Novosel, Nives ; Ivošević DeNardis, Nadica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The book of Elecnano9 / - , 2020, 112-112

Skup
9th meeting of Electrochemistry in Nanoscience

Mjesto i datum
Pariz, Francuska, 23.11.2020. - 24.11.2020

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
adhesion ; algae ; interface ; mechanical properties ; cell barrier

Sažetak
Fundamental understanding of adhesion behavior of algal cells at the model charged interface is of relevance for cell fusion, cell activity, and direct studies of non-specific interactions between living cells. Mercury electrode is a commonly used substrate for cell adhesion studies due to atomically smooth, chemically inert, conductive surface, and large set interfacial data. Attractive interaction between a single cell and the model charged interface is controlled by applied potentials using chronoamperometry. The adhesion of single cells causes a displacement of counter-ions from the electrical double layer. The instant flow of compensating current (amperometric signal) reflects the dynamics of adhesive contact formation and the spreading of a cell (1). We aim to examine the effect of cell barrier structure on adhesion and spreading of unicellular algal species at the model charged interface. As model organisms, four unicellular algal cell species were chosen. Results show that Dunaliella tertiolecta and Prorocentrum micans adhere to the species-specific potential range, and their amperometric signal amplitude is in line with cell size. Tetraselmis suecica and Cylindrotheca closterium do not adhere to the model charged interface behaving as an inert particle. The differences in adhesion behavior are in agreement with the reported mechanical properties of the cell barrier. Dunaliella cells are enclosed within a thin elastic plasma membrane with a glycocalyx surface coat and determined cell elasticity is in a tenth of kPa (2). Prorocentrum cells possess cellulose amphiesma. Tetraselmis cells are encased within a thin calcite incrustrated theca, while Cylindrotheca cells possess a rigid organosilicate cell wall with the elasticity up to 20 MPa (3). Electrochemical adhesion-based differentiation identified in the algal cell systems could be relevant for industrial processes enabling fast characterization of soft cell species and differentiation from the rigid cell species without sample pretreatment.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Interdisciplinarne prirodne znanosti, Biotehnologija

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