Naslov
CRKL GENE EXPRESSION IN KIDNEYS OF YOTARI MICE

Autori
Racetin, Anita ; Lozić, Mirela ; Jurić, Marija ; Šolić, Ivana ; Kosović, Ivona ; Kolobarić, Anita ; Barbarić, Maja ; Cloarec, Melissa ; Filipović, Natalija ; Saraga-Babić, Mirna ; Vukojević, Katarina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Skup
1st Congress of Geneticists in Bosnia and Herzegovina with International Participation

Mjesto i datum
Sarajevo, Bosna i Hercegovina, 02.10.2019. - 04.10.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
CRKL, yotari mouse, kidney development

Sažetak
The aim of this study was to explore difference in spatial expression pattern of CrkL protein in the kidney tissue between wild type (Dab1+/+), heterozygotes (Dab1+/- ) and yotari (Dab1 -/- ) mice to determine its possible roles in maintenance of kidney function. Yotari mouse is constructed by PGK-neo cassette that cause target disruption of the first 47 codons of the gene coding for protein-interacting (PI-PTB) domain. Heterozygotes (Dab1 +/- ) were mated and three groups of pups were observed in this study, Dab1 -/- or yotari group, Dab1 +/- and Dab1+/+ or control group. Pups were sacrificed four days after birth and samples were embedded in the paraffin. CrkL expression was examined in the kidney sections of control and yotari mice by immunofluorescence staining. The percentage of CrkL immunoreactive cells were calculated. For each investigated period we captured at least twenty images per different kidney structure: proximal convoluted tubules (PCT), distal convoluted tubules (DCT) and glomeruli at 40× objective magnification. Any level of cytoplasmic or membrane staining with used marker was regarded as positive. Data were analysed by student (t) test in Past3 statistic program. Statistical significance was considered at P<0.05. CrkL was moderately expressed in kidneys of yotari mice and occasionally in heterozygotes with specific spatial expression pattern and diverse fluorescence intensity. Dab1+/+ kidneys contain almost no staining. Expression in DCT of the yotari mice significantly increased (P<0.05) in comparison to expression in DCT of Dab1 +/- and Dab1+/+ mice. There was no statistically significant difference in CrkL expression in PCT and glomeruli between any group of mice. Increased expression of CrkL protein in DCT of yotari mice implies its potentially significant role in kidney damage.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA