Pregled bibliografske jedinice broj: 109373
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu // Zbornik radova V. Simpozija Hrvatskog društva za zaštitu od zračenja / Krajcar-Bronić, Ines ; Miljanić, Saveta ; Obelić, Milko (ur.).
Zagreb, 2003. str. 151-155 (predavanje, domaća recenzija, cjeloviti rad (in extenso), znanstveni)
CROSBI ID: 109373 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Niske doze gama zračenja smanjuju osjetljivost tumorskih stanica grlića maternice na cisplatinu
(Low doses of gamma rays reduce the sensitivity of cervical carcinoma cells to cisplatin)
Autori
Osmak, Maja ; Brozović, Anamaria
Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni
Izvornik
Zbornik radova V. Simpozija Hrvatskog društva za zaštitu od zračenja
/ Krajcar-Bronić, Ines ; Miljanić, Saveta ; Obelić, Milko - Zagreb, 2003, 151-155
Skup
V. Simpozij Hrvatskog društva za zaštitu od zračenja sa međunarodnim učešćem
Mjesto i datum
Stubičke Toplice, Hrvatska, 09.04.2003. - 11.04.2003
Vrsta sudjelovanja
Predavanje
Vrsta recenzije
Domaća recenzija
Ključne riječi
gama zračenje; apoptoza; stanice
(gamma rays; apoptosis; cells)
Sažetak
One of the major challenges of modern genetics is to apply recent advances in mutation research to improve the accuracy of the estimates of the genetic risk for humans. Because of the important implications for radiation protection, biological effects of low-dose radiation have been a focus of research in recent years. Previously we have found that human cervical carcinoma HeLa cells irradiated repeatedly with low doses of gamma rays (HeLa1500 cells) became resistant to cisplatin. In this study we examine whether this effect was caused by inhibition of apoptosis. In HeLa and HeLa1500 cells we determined the induction of apoptosis following the treatment with cisplatin (i) by counting apoptotic cells with characteristic morphological changes, (ii) by analysing the expression of apoptotic genes involved in cytochrome c/Apaf-1/caspase-9 and in Fas/FasL pathways by Western blot method, and (iii) by estimating the activities of caspases by commercial caspase detection kits. Our results show that low doses of gamma rays induced alterations in human cervical carcinoma cells that were reflected in inhibition of p53-independent cisplatin-induced apoptosis due to reduced activity of caspase 3.
Izvorni jezik
Hrvatski
Znanstvena područja
Biologija
