Naslov
Determination of volatile aromatic hydrocarbons and their degradation metabolites in human urine as an indicator of the state of the environment

Autori
Aleksandar Bulog

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, prošireni sažetak, znanstveni

Izvornik
International days of public and environmental health profession 2020 / Krulec A. ; Tajnikar S. ; Ovca A. - Ljubljana : Inštitut za sanitarno inženirstvo, Institute of Public and Environmental Health, Zaloška cesta 155, SI-1000 Ljubljana, Slovenija, 2020, 17-17

ISBN
978-961-94556-6-1

Skup
International days of public and environmental health profession 2020

Mjesto i datum
Ljubljana, Slovenija, 22.10.2020. - 23.10.2020

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Aromatic hydrocarbons, S-phenyl mercapturic acid, S-benzyl mercapturic acid

Sažetak
This study covered the determination of benzene, toluene, ethylbenzene and xylene isomers as characteristic volatile aromatic hydrocarbons related to the environmental impact on human health.The chosen method for this research is the gas chromatography method based on solid phase analyte microextraction (HS-SPME).The study also reported a method to simultaneously determine S-phenyl mercapturic acid (S-PMA) and S-benzyl mercapturic acid(S-BMA) in human urine. Since constant exposure to benzene isomers it is necessary to quantify this issue. S-PMA is believed to be a sensitive and specific biomarker for evaluating benzene exposure at low levels but also a metabolite of sorbic acid. Specific metabolite of toluene is excreted in urine in the form of S-BMA. Urine samples were analyzed by ultra-highperformance liquid chromatography/tandem mass spectrometer in negative electrospray ionization mode. To perform matrix match calibration standards were prepared in synthetic urine. Target analytes eluted within 15 min in multiple reaction monitoring mode. In order to achieve maximum sensitivity instrument conditions were automatically optimized. Urine samples were refrigerated until analysis and stability was observed within 15 days. We can conclude that the selected methods represent a quality analytical tool for determining the concentrations of volatile aromatic hydrocarbons and their degradation metabolites in human urine.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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