Naslov
Control Mechanisms for Cucurbita pepo L. Somatic Embryogenesis

Autori
Leljak-Levanić, Dunja ; Bauer, Nataša ; Mihaljević, Snježana ; Jelaska, Sibila

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3rd Croatian Scientific Conference about Biotechnology - Biotechnology and Food - program and abstracts / Kniewald, Zlatko - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2003

Skup
3rd Croatian Scientific Conference about Biotechnology - Biotechnology and Food

Mjesto i datum
Zagreb, Hrvatska, 17.02.2003. - 20.02.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Sažetak
In order to apply plant biotechnology strategies to the pumpkin breeding it is necessary to obtain reliable procedures for plant regeneration from cell or tissue culture. The formation of adventitious shoot buds and/or somatic embryos depend on the plant species and on in vitro manipulation. In pumpkin (Cucurbita pepo L.) somatic embryogenesis was established on 2, 4-D containing MSC medium and on hormone-free MS medium with modified nitrogen sources (NH4Cl was a sole source of nitrogen). Embryo development of three pumpkin lines was controlled with 2, 4-D addition/removal and by changing in reduced/unreduced nitrogen ratio. A correlation among factors controlling the embryo development (2, 4-D and nitrogen sources) and callose deposition, extracellular (glyco)proteins and DNA methylation has been demonstrated. Reduced nitrogen was associated with high level of DNA methylation, callose accumulation in cell walls, lack of protein secretion and developmental blockage in globular stage of embryos. Unreduced nitrogen in MSC medium enabled embryo development to torpedo (on 2, 4-D supplemented MSC) and to cotyledonary stages (on 2, 4-D-free MSC). The embryo maturation corresponded with degradation of callose, secretion of specific set of glyco(proteins) and with decreasing of DNA methylation. Tunicamycin affected the glycosylation of some glycoproteins and 5-azacytidine decreased DNA methylation, however they did not prevent the embryogenesis.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA