Naslov
Clinical interpretation of serologic tests in diagnostic procedures in toxoplasmosis

Autori
Vranić-Ladavac, Mirna ; Markotić, Alemka ; Pfeifer, Dina ; Ladavac, Ranko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstract book / - , 2002, 59-60

Skup
3rd Croatian Congress on Infectious Diseases with international participation

Mjesto i datum
Dubrovnik, Hrvatska, 12.10.2002. - 15.10.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Sažetak
Toxoplasma gondii infection has world wide distribution and cause chronic infection in > 10^9 people. Prevalence varies betwen 10 to 60% in different countries (Croatia 31-50 %). Toxoplasmosis is a benign disease in immuno-competent persons, but in immuno-compromised individuals (especially congenitally infected infants, organ transplant recipients and AIDS patients) may cause life-threathing complications. The determination of diagnosis and therapy on the basis of a single serum examination is very important ; posible on the basis of a single serum sample. Therefore it is of great importance to confirm particular antibody classes using a combination of suitable serological tests and so in most cases it is possible to differentiate between recent and latent infections. We suggest diagnostic procedures for 4 groups of patients: pregnant, neonated with suspected congenital toxoplasmosis, immunodeficient patients and immunocompentent patients. The diagnostic methods consist of combination of basic and supplemented tests. Basic tests include the detection of specific classes of IgM and IgG antibodies using ELISA or IFA tests. When necessary activity of toxoplasma infection can be determined by supplementary methods of e.g. IgA antibody (ISAGA ; ELISA), and new tests IgM SAG1 (ELISA microcapture) and IgG avidity (ELISA ; ELFA). In special cases several complementary detection methods can be envisaged like detection of the parasite (e.g.cell culture using fibroblasts or monocytoid cells), detection of antigens and detection of parasite DNA. We try to present interpretations of suspected cases including proposals for clinicians. We have most clinical experiences with diagnostic procedures for acute toxoplasmosis in pregnat women . Between May/2000 and May/2001 from a resident population of 200.000, 960 sera samples from 937 pregnant women were analysed for presence of Toxsoplasma gondi specific IgM and IgG antibodies. IFA and ELISA test were performed and 32% of samples were positive for presence of Toxoplasma gondii IgG antibodies. In 7 pregnant women (0.74%) IgM and IgG antibodies both were identified. IgG avidity test indicated high avidity in four women, thereby excluding the acute primary infection. In the other three with low IgG avidity artificial abortion was performed in one, and macrolide therapy initiated in remaining two pregnanci es with diagnosed acute infection (All assays BioMérieux, France). Toxoplasma gondii.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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