Naslov
Chromatographic Detection of 8-Hydroxy-2'- Deoxyguanosine in Leukocytes of Asbestos Exposed Workers for Assessing Past and Recent Carcinogen Exposures

Autori
Cellai, Filippo ; Bonassi, Stefano ; Cristaudo, Alfonso ; Bonotti, Alessandra ; Neri, Monica ; Ceppi, Marcello ; Bruzzone, Marco ; Milić, Mirta ; Munnia, Armelle ; Peluso, Marco

Izvornik
Diagnostics (2075-4418) 10 (2020), 4; 239, 12

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
asbestos ; leukocytes ; 8-oxodG

Sažetak
Asbestos fibers include a group of silicate minerals that occur in the environment and are widely employed in occupational settings. Asbestos exposure has been associated to various chronic diseases ; such as pulmonary fibrosis ; mesothelioma ; and lung cancer ; often characterized by a long period of latency. Underlying mechanisms that are behind the carcinogenic e ect of asbestos have not been fully clarified. Therefore ; we have conducted an epidemiological study to evaluate the relationship between 8-hydroxy-20- deoxyguanosine (8-oxodG), one of the most reliable biomarkers of oxidative stress and oxidative DNA damage ; and asbestos exposure in the peripheral blood of residents in Tuscany and Liguria regions ; Italy ; stratified by occupational exposure to this carcinogen. Levels of 8-oxodG were expressed such as relative adduct labeling (RAL) ; the frequency of 8-oxodG per 105 deoxyguanosine was significantly higher among exposed workers with respect to the controls ; i.e., 3.0  0.2 Standard Error (SE) in asbestos workers versus a value of 1.3  0.1 (SE) in unexposed controls (p < 0.001). When the relationship with occupational history was investigated ; significant higher levels of 8-oxodG were measured in current and former asbestos workers vs. healthy controls ; 3.1  0.3 (SE) and 2.9  0.2 (SE), respectively. After stratification for occupational history ; a significant 194% excess of adducts was found in workers with 10 or more years of past asbestos exposure (p < 0.001). 8-oxodG can be used for medical surveillance programs of cohorts of workers with past and recent exposures to carcinogens for the identification of subjects requiring a more intense clinical surveillance.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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