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Pregled bibliografske jedinice broj: 1089945

Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Møller, Peter; Azqueta , Amaya: Boutet-Robinet, Elisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, Solange; Teixeira, João Paulo; Costa Pereira, Cristiana; Dusinska, Maria et al.
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results // Nature Protocols, 15 (2020), 12; 1-10 doi:10.1038/s41596-020-0398-1. (međunarodna recenzija, pregledni rad, znanstveni)

CROSBI ID: 1089945 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results

Autori
Møller, Peter ; Azqueta , Amaya: Boutet-Robinet, Elisa ; Koppen, Gudrun ; Bonassi, Stefano ; Milić, Mirta ; Gajski, Goran ; Costa, Solange ; Teixeira, João Paulo ; Costa Pereira, Cristiana ; Dusinska, Maria ; Godschalk, Roger ; Brunborg, Gunnar ; Gutzkow B, Kristine ; Giovannelli, Lisa ; Cooke, Marcus S ; Richling, Elke ; Laffon, Blanca ; Valdiglesias, Vanessa ; Basaran, Nursen ; Del Bo', Cristian ; Zegura, Bojana ; Novak, Matjaz ; Stopper, Helga ; Vodicka, Pavel ; Vodenkova, Sona ; de Andrade Moreas, Vanessa ; Sramkova, Monika ; Gabelova, Alena ; Collins, Andrew ; Langie, Sabine

Izvornik
Nature Protocols (1754-2189) 15 (2020), 12; 1-10

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, pregledni rad, znanstveni

Ključne riječi
alkaline comet assay parameters ; enzyme modified alkaline comet assay parameters

Sažetak
The comet assay is a widely used test for the detection of DNA damage and repair activity. However, there are interlaboratory differences in reported levels of baseline and induced damage in the same experimental systems. These differences may be attributed to protocol differences, although it is difficult to identify the relevant conditions because detailed comet assay procedures are not always published. Here, we present a Consensus Statement for the Minimum Information for Reporting Comet Assay (MIRCA) providing recommendations for describing comet assay conditions and results. These recommendations differentiate between ‘desirable’ and ‘essential’ information: ‘essential’ information refers to the precise details that are necessary to assess the quality of the experimental work, whereas ‘desirable’ information relates to technical issues that might be encountered when repeating the experiments. Adherence to MIRCA recommendations should ensure that comet assay results can be easily interpreted and independently verified by other researchers.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Javno zdravstvo i zdravstvena zaštita, Veterinarska medicina, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Ustanove:
Institut za medicinska istraživanja i medicinu rada, Zagreb

Profili:

Avatar Url Goran Gajski (autor)

Avatar Url Mirta Milić (autor)

Poveznice na cjeloviti tekst rada:

Pristup cjelovitom tekstu rada doi www.nature.com

Citiraj ovu publikaciju:

Møller, Peter; Azqueta , Amaya: Boutet-Robinet, Elisa; Koppen, Gudrun; Bonassi, Stefano; Milić, Mirta; Gajski, Goran; Costa, Solange; Teixeira, João Paulo; Costa Pereira, Cristiana; Dusinska, Maria et al.
Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results // Nature Protocols, 15 (2020), 12; 1-10 doi:10.1038/s41596-020-0398-1. (međunarodna recenzija, pregledni rad, znanstveni)
Møller, P., Azqueta , Amaya: Boutet-Robinet, Elisa, Koppen, G., Bonassi, S., Milić, M., Gajski, G., Costa, S., Teixeira, J., Costa Pereira, C. & Dusinska, M. (2020) Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results. Nature Protocols, 15 (12), 1-10 doi:10.1038/s41596-020-0398-1..
@article{article, author = {M\oller, Peter and Koppen, Gudrun and Bonassi, Stefano and Mili\'{c}, Mirta and Gajski, Goran and Costa, Solange and Teixeira, Jo\~{a}o Paulo and Costa Pereira, Cristiana and Dusinska, Maria and Godschalk, Roger and Brunborg, Gunnar and Gutzkow B, Kristine and Giovannelli, Lisa and Cooke, Marcus S and Richling, Elke and Laffon, Blanca and Valdiglesias, Vanessa and Basaran, Nursen and Del Bo', Cristian and Zegura, Bojana and Novak, Matjaz and Stopper, Helga and Vodicka, Pavel and Vodenkova, Sona and de Andrade Moreas, Vanessa and Sramkova, Monika and Gabelova, Alena and Collins, Andrew and Langie, Sabine}, year = {2020}, pages = {1-10}, DOI = {10.1038/s41596-020-0398-1.}, keywords = {alkaline comet assay parameters, enzyme modified alkaline comet assay parameters}, journal = {Nature Protocols}, doi = {10.1038/s41596-020-0398-1.}, volume = {15}, number = {12}, issn = {1754-2189}, title = {Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results}, keyword = {alkaline comet assay parameters, enzyme modified alkaline comet assay parameters} }
@article{article, author = {M\oller, Peter and Koppen, Gudrun and Bonassi, Stefano and Mili\'{c}, Mirta and Gajski, Goran and Costa, Solange and Teixeira, Jo\~{a}o Paulo and Costa Pereira, Cristiana and Dusinska, Maria and Godschalk, Roger and Brunborg, Gunnar and Gutzkow B, Kristine and Giovannelli, Lisa and Cooke, Marcus S and Richling, Elke and Laffon, Blanca and Valdiglesias, Vanessa and Basaran, Nursen and Del Bo', Cristian and Zegura, Bojana and Novak, Matjaz and Stopper, Helga and Vodicka, Pavel and Vodenkova, Sona and de Andrade Moreas, Vanessa and Sramkova, Monika and Gabelova, Alena and Collins, Andrew and Langie, Sabine}, year = {2020}, pages = {1-10}, DOI = {10.1038/s41596-020-0398-1.}, keywords = {alkaline comet assay parameters, enzyme modified alkaline comet assay parameters}, journal = {Nature Protocols}, doi = {10.1038/s41596-020-0398-1.}, volume = {15}, number = {12}, issn = {1754-2189}, title = {Minimum Information for Reporting on the Comet Assay (MIRCA): recommendations for describing comet assay procedures and results}, keyword = {alkaline comet assay parameters, enzyme modified alkaline comet assay parameters} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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