Naslov
New insights into bacterial phosphoproteome analysis

Autori
Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
Summer Schools in Applied Molecular Microbiology: Microbial Metabolites in Nature and Medicine

Mjesto i datum
Dubrovnik, Hrvatska, 25.08.2012. - 02.09.2012

Vrsta sudjelovanja
Pozvano predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Phosphoproteome, Serine, Threonine and Tyrosine phosphorylation, Bacteria

Sažetak
Protein phosphorylation occurs in all organisms and possesses crucial regulatory roles in a broad spectrum of biological processes. It was discovered in the mid 1950s and for many years it was tought to exist only in eukaryotes. Serine, threonine and tyrosine phosphorylation is the most common type of phosphorylation in eukaryotes, on contrary, in bacteria phosphorylation occurs predominantly on histidine and aspartate (TCS). Until the early 1990s it was largely considered that these two phosphorylation systems are mutually exclusive. Genome sequencing confirmed the widespread presence of genes encoding eukaryotic like Ser/Thr kinases and phosphatases in bacteria. A global, gel-free approach in combination with biochemical enrichment and the use of a high accuracy mass spectrometry allowed identification of numerous proteins phosphorylated on Ser/Thr/Tyr in different bacterial species indicating that this type of phosphorylation is widespread in bacteria. Detected phosphoproteins are involved in a wide variety of metabolic processes which strongly supports the emerging view that protein phosphorylation is a general and fundamental regulatory process not restricted only to eukaryotes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA