Naslov
HPLC analysis of citric and tartaric acids in fruit nectars and juices

Autori
Inić, Suzana ; Ljepović, Maja ; Domijan, Ana- Marija ; Steiner Srdarević, Sabina ; Jablan, Jasna ; Markov, Ksenija

Izvornik
Croatica chemica acta (0011-1643) 93 (2020), 1; 57-62

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
citric acid ; tartaric acid ; fruit juices ; monitoring ; green chemistry ; Croatian market

Sažetak
The aim of this study was to estimate a level of citric and tartaric acids in fruit nectars (n = 17) and juices (n = 13) available on the Croatian market. For analysis reverse-phase HPLC with UV/Vis detector set at 214 nm was used. Mobile phase was phosphate buffer (50 mM, pH = 2.80) at flow rate 0.5 mL min–1. The method was linear (r2 = 0.9999). LoD was 0.01 g L–1, LoQ was 0.03 g L–1 and the intra-day along with the inter-day variability were up to 3 %. The level of citric and tartaric acids in fruit nectars ranged from 1.26 to 4.42 g L–1 and 0.68 to 0.86 g L–1, respectively, and in fruit juices ranged from 3.03 to 7.67 g L–1 and 3.09 to 4.68 g L–1, respectively. A higher level of citric acid in fruit juices than in fruit nectars was detected (p < 0.05 ; MannWhitney U test). Six fruit juices contained a higher level of citric acid allowed by EU regulation implying the importance of monitoring concentrations of both acids in food products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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