Naslov
Review and analysis of measurements of total phenolic contents in honey samples

Autori
Drago, Bešlo ; Lučić, Bono

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts (Ružička days) / Jukić, Ante - Zagreb : Osijek : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2020, 127-127

ISBN
978-953-6894-75-8

Skup
18. Ružičkini dani "Danas znanost - sutra industrija"

Mjesto i datum
Vukovar, Hrvatska, 16.09.2020. - 18.09.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
honey ; total polyphenols ; spectrophotometric method

Sažetak
Antioxidants play an important role in preserving human health in the fight against cell damage caused by free radicals [1]. Different types of honey and from different geographical origins show different polyphenol content. The aim of this study was to determine the total polyphenol content (TPC) in 96 honey samples from different countries of Central and Eastern Europe (Croatia, Serbia, Bosnia and Herzegovina, Hungary, Slovenia and Italy) by a spectrophotometric method with Folin Ciocalteu reagent [2]. If we analyze the measurements of the same types of honey (e.g. acacia or chestnut) from a number of literature sources, large differences in the values of total phenols are observed, which cannot be attributed only to seasonal or geographical variations. Our previous studies gave the TPC values from 1164.99 mg/kg to 212.9 mg/kg for the dominant uni- and multi-floral honey. In an attempt to decipher the reasons for these excessive differences, we analyzed the impact of sample ageing and seasonal and geographic variations. However, some differences are too large, and the cause seems to be the inconsistency of the calculation of the measurement units. Namely, some authors state that they modified method [1, 2], but do not state in detail which part they modified. Our experience to date shows that the authors took different amounts of honey for analysis and some used a gallic acid for calibration curve in the range of 0–200 μg/mL and some of 0–400 μg/mL. For these reasons, we would suggest a more strict standardisation of the total polyphenol content measurements.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Farmacija

POVEZANOST RADA