Naslov
Copper - Phospholipid Interaction at Cell Membrane Model Hydrophobic Surfaces

Autori
Mlakar, Marina ; Cuculić, Vlado ; Frka Milosavljević, Sanja ; Gašparović, Blaženka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
On-line book of abstracts / - , 2018, 1265-1265

Skup
2018 Ocean science meeting

Mjesto i datum
Portland (OR), Sjedinjene Američke Države, 11.02.2018. - 16.02.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Copper ; phospholipid ; interaction ; model membrane

Sažetak
Interactions of micronutrient metal ions with cells, primarily membranes, in the marine environment are of particular interest as they are crucial for cell function maintenance. Micronutrient metal resources are frequently limited and marine organisms develop different mechanisms for their consumption, which mostly allude their complexation with organic ligands to make micronutrient bioavailable. It is highly important to understand possible mechanism of acquisition of micronutrients to the cells. The aim of this study was to examine copper (II) interaction mechanism with lipids as vital part of cell membrane, for the first time. Investigation of Cu (II) binding with natural lipid phosphatidylglycerol (PG) in aquatic solution is carried out by voltammetric measurements at the mercury drop electrode used as model for hydrophobic cell membrane. Results are supported by monolayer studies in a Langmuir trough and zeta-potential measurements by dynamic light scattering (DLS) in combination with an applied electic field (electrophoresis). Penetration of Cu2+ ions into the PG layer is enabled by formation of hydrophilic Cu2+ - Phenanthroline (Phen) complex in the subphase, followed by mixed ligand complex Cu-Phen-PG formation at the hydrophobic interface. Cu-Phen-PG was identified in pH range from 6 to 9. Stoichiometry of the complex ([PhenCuOHPG]), as well as its stability and kinetics were determined at the mercury drop electrode.

Izvorni jezik
Engleski

Znanstvena područja
Interdisciplinarne prirodne znanosti

POVEZANOST RADA