Naslov
Mass spectrometry approach for analysing protein phosphorylation in antibiotic producing bacteria

Autori
Šarić, Ela ; Quinn, Gerre ; Šemanjski, Maja ; Paradžik, Tina ; Jurin, Mladenka ; Hunter, Iain ; Herron, Paul ; Maček, Boris ; Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of Molecular Biophysics: ABC of the puzzle of life / - , 2019, 133-134

Skup
3rd COST-sponsored ARBRE-MOBIEU plenary meeting Molecular Biophysics - ABC of the puzzle of Life

Mjesto i datum
Zagreb, Hrvatska, 18.03.2019. - 20.03.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
phosphorylation ; S. rimosus ; OTC production ; mass spectrometry

Sažetak
Streptomyces is the largest genus of Actinobacteria, bacteria that exibit complex and tightly regulated life cycle. These bacteria are best known for their ability to produce many bioactive metabolites of medical and biotechnological importance. Approximately 75% of commercially and medically useful antibiotics are produced by different Streptomyces species. Antibiotic production generally coincides with the onset of morphological differentiation. Streptomyces often exibit growth arrest in the early exponential phase, so-called ‘’decision’’ phase, which is important for antibiotic production. During the course of this study, growth arrest was observed in different S. rimosus strains. This species is among the best-characterised industrial Streptomyces known to produce oxytetracycline (OTC). Phosphorylation plays critical roles in the regulation of many cellular processes. The aim of this work was to uncover possible regulatory role of protein phosphorylation in OTC production. Analysis of the S. rimosus genome has predicted 33 eukaryotic-like protein kinases and 27 eukaryotic type protein phosphatases. Western blot confirmed activity of Ser/Thr/Tyr kinases in cell free extracts collected throughout the growth of six S. rimosus strains. Selected strains produced significantly different levels of OTC. For this study we selected the best OTC producer, strain 23383, and the wild type, strain G7. Taking into account the importance of protein phosphorylation in the regulation of antibiotic production in other bacteria, we had two main objectives. First was to examine the differences in phosphoproteome of OTC producing bacteria at different stages of growth. The second was to find the differences between phosphoproteome of two strains that produce significantly different amounts of antibiotic. The results of comparative proteome/phosphoproteome analysis pointed out several proteins which may be important for the OTC production and responsible for the bacterial resistance to elevated concentration of the antibiotic in the producing media.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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