Naslov
Dynamics of protein phosphorylation in S. rimosus - oxytetracycline producer strains

Autori
Šarić, Ela ; Quinn, Gerre ; Šemanjski, Maja ; Paradžik, Tina ; Jurin, Mladenka ; Hunter, Iain ; Herron, Paul ; Maček, Boris ; Vujaklija, Dušica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Ljetnja škola / - , 2018, No-no

Skup
John Innes/Rudjer Bošković Summer School on “Microbial Diversity and Specialised Metabolites”

Mjesto i datum
Dubrovnik, Hrvatska, 08.09.2018. - 16.09.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
streptomycetes ; phosphorylation ; S. rimosus ; OTC production

Sažetak
Streptomycetes are multicellular, differentiated microorganisms particularly interesting for their ability to produce a plethera of bioactive secondary metabolites, among which the best known are antibiotics. Indeed, about 75% of commercially and medically useful antibiotics are produced by different Streptomyces species. Antibiotic production generally coincides with the onset of morphological differentiation. In S. griseus growth arrest in the early exponential phase, also called ''decision'' phase is important growth stage for antibiotic production. During the course of this study, the existance of the same phase was observed in different S. rimosus strains. This species is among the best- characterised industrial streptomycetes known to produce oxytetracycline (OTC). Phosphorylation plays critical roles in the regulation of many cellular processes. The aim of this work was to uncover the role of protein phosphorylation in OTC production. Analysis of the S. rimosus genome has predicted 33 eukaryotic-like protein kinases and 27 eukaryotic type protein phosphatases. Western blot analysis showed intensive activity of Ser/Thr/Tyr kinases in cell free extracts collected throughout the growth of six S. rimosus strains. Selected strains produced significantly different levels of OTC. For this study we selected the best OTC producer, strain 23383, and the wild type, strain G7. Taking into account the importance of protein phosphorylation in the regulation of antibiotic production, we posted two main questions. First, what are the differences in phosphoproteome of OTC producing bacteria at different stages of growth? Second, what are the differences between phosphoproteome of two strains that differ significantly in antibiotic production? In order to adress these questions, we investigated comparative proteome/phosphoproteome of selected using high accuracy mass spectrometry in combination with biochemical enrichment of phosphopeptides.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA