Naslov
Interaction of 3H-aldosterone with rat kidney plasma membranes

Autori
Ožegović, Božena ; Schön, Elizabeta ; Milković, Stevan

Izvornik
Journal of steroid biochemistry (0022-4731) 8 (1977), 8; 815-819

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
3H-aldosterone ; rat kidney plasma membrane ; steroid receptors

Sažetak
The interaction of 3H-aldosterone with isolated rat kidney plasma membranes has been studied by centrifugation and gel filtration methods. The Scatchard plot analysis of data indicated the affinity of aldosterone for membrane sites, expressed as equilibrium constant Kdiss, to be 1, 3 x 10^-8 M and the concentration of binding sites (n) 1, 69 x 10^-13 mol/mg protein. The high concentration of unlabelled aldosterone present in the incubation medium, or injected into rats 45 min before killing, decreased the amount of labelled aldosterone bound to plasma membranes. Gel filtration on Sephadex G-200 and disc electrophoresis on polyacrylamide gel of solubilized 3H-aldosterone-renal plasma membranes complex showed that the hormone was bound to the high molecular weight protein component of the plasma membranes. The proteinaceous nature of membrane component which bound aldosterone was determined using delipidated plasma membranes.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA