Naslov
Lifelong changes in cingulate cortex and learning behavior after moderate perinatal hypoxia in the rat

Autori
Trnski, Sara ; Ilić, Katarina ; Nikolić, Barbara ; Darmopil, Sanja ; Petanjek, Zdravko ; Hranilović, Dubravka ; Jovanov Milošević, Nataša

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
FENS 2020 Virtual Forum

Mjesto i datum
Online ; Glasgow, Ujedinjeno Kraljevstvo, 11.07.2020. - 15.07.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Recenziran

Ključne riječi
hypoxia ; brain development ; perineuronal nets ; learning

Sažetak
Aiming to find molecular, cellular, and structural brain hallmarks and related behavioral outcome after perinatal hypoxia, we designed a non- invasive model of perinatal hypoxia in the newborn rats. In the randomized study, fifty-two Wistar Han rats were subjected to general hypoxia (pO2 73mmHg/2h) or normoxia, 24h after birth. The Western blot method applied 2h, 8h, 12h, and 24h later, showed a gradual increase of the hypoxia- inducible factor 1α, and a decrease of the cytochrome-C- oxygenase (subunit 4, isoform 1) expression, revealing moderate brain lesion in the rats subjected to hypoxia. The behavioral testing at 30th and 70th postnatal days, using open field, hole board, social choice, and T-maze paradigm displayed significant hyperactivity and slower pace of learning in the rats that were exposed to perinatal hypoxia. The closer examination of the cingulate cortex at the age of 4 months, by WFA- and anti-parvalbumin immunohistochemistry, disclosed a significantly increased number of perineuronal nets and parvalbumin-interneurons in the medial and retrosplenial areas. Thus, the moderate hypoxia in the newborn rats causes lifelong changes at the molecular and connectivity level in the cingulate cortex and related behavioral outcome. This new, non- invasive perinatal brain lesion in rats, according to the findings obtained, successfully resembled a frequent but diagnostically neglected moderate perinatal brain injury in humans and may establishes as a complementary model for future search for prevention, diagnostic and therapeutic strategies for perinatal brain injuries in human.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA