Naslov
Production of biodisel from microbial lipids

Autori
Grubišić, Marina ; Galić, Maja ; Ivančić, Šantek, Mirela ; Šantek, Božidar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Proceedings of NATURAL RESOURCES, GREEN TECHNOLOGY AND SUSTAINABLE DEVELOPMENT-GREEN/3 / - , 2018, 109-109

Skup
3rd Natural resources green technology & sustainable development-GREEN/3

Mjesto i datum
Zagreb, Hrvatska, 05.06.2018. - 08.06.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biodiesel, corn cobs, lignocellulosic biomass, microbial lipids, Mortierella isabellina

Sažetak
Corn cob hydrolysate was used as carbon source for microbial lipids production by the oleaginous fungus Mortierella isabellina. In order to improve the enzymatic digestibility, corn cobs were subjected to alkaline pretreatment with NaOH and acid pretreatment with H2SO4. Pretreatment process was carried out at different NaOH and H2SO4 concentrations, temperature and residence time. Optimal pretreatment conditions were chosen based on glucose and xylose yield by enzymatic hydrolysis of pretreated biomass. The highest efficiency of enzymatic hydrolysis was obtained with corn cobs pretreated with 2 % w/w NaOH at 121oC and residence time of 1 hour, 2 % w/w NaOH at 50oC and residence time of 6 hours and 1 % w/w H2SO4 at 121oC and residence time of 20 min. Furthermore, the effect of enzyme and substrate loading rate on efficiency of enzymatic hydrolysis of pretreated corn cobs was also studied. The highest efficiency of glucan and xylan hydrolysis was achieved at 20 % (g/g) substrate loading rate and 30 FPU g-1 of enzyme loading rate. Oleaginous fungus Mortierella isabellina was cultivated on the enzymatic hydrolysate of corn cobs pretreated with 2 % w/w NaOH at 50 oC for 6 hours. The microbial lipid content in fungal biomass reached up to 45 % of biomass dry weight, while lipid productivity and lipid concentrations were 0, 051 gL-1h-1 and 24, 21 gL- 1, respectively.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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