Naslov
Process development for Escherichia coli based pyruvate production

Autori
Zelić, Bruno ; Takors, Ralf ; Vasić-Rački, Đurđa ; Wandrey, Christian

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Sažetci / Vicković, Ivan - Koprivnica : HKD i HKDI, 2001

Skup
XVII. Hrvatski skup kemičara i kemijskih inženjera

Mjesto i datum
Osijek, Hrvatska, 10.06.2001. - 13.06.2001

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
E. coli; process development; pyruvate

Sažetak
The commercial demand for pyruvic acid has been expanding because of its use as an effective starting material for the synthesis of many drugs, agrochemicals and nowadays in food industry as a fat burner. It is also a valuable substrate for the enzymatic production of amino acids such as L-tryptophan, L-tyrosine and L-dihydroxyphenylalanine (L-DOPA). A pyruvate production process from glucose and acetate by Escherichia coli acetate auxotroph strain* was investigated in different reactors (batch, fed-batch, continuous) at varied process conditions and fermentation mediums. Biomass concentration is increased more than 10 % and space-time yield is increased 20 % by using simple acetate feeding based on off-line biomass measurement and off-line specific biomass growth rate calculation in comparison with the batch process. Using a Pan medium instead of M9 medium a 40% increase in biomass concentration with same space-time yield was achieved as compared to the case before. Limiting the amount of glucose feed together with acetate (by using same acetate feeding strategy described before) final biomass concentration was 100 % higher and space-time yield was 50 % higher. To prevent acetate limitation and to keep constant level of acetate in fermentation medium connection between CO2 transfer rate (CTR) and acetate consumption rate was detected which will be used for on-line calculation of acetate feed (open-loop regulation). On-line general analyzer (OLGA) for glucose concentration measurement will be used for on-line calculation of glucose feed (closed-loop control) to ensure unlimited conditions in the process concerning glucose. *Strain was developed in Institute of Biotechnology 1, Forschungszentrum Jülich GmbH. Work is a part of research of DBU project 13040/05.

Izvorni jezik
Engleski

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