Naslov
Fed-batch Process for Pyruvate Production by Recombinant Escherichia coli YYC202 strain

Autori
Zelić, Bruno ; Gerharz, Tanja ; Bott, Michael ; Vasić-Rački, Đurđa ; Wandrey, Christian ; Takors, Ralf

Izvornik
Chemical Engineering and Technology (0930-7516) 26 (2003), 3; 299-305

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Escherichia coli; Pyruvate

Sažetak
Fed-batch fermentation was applied to the production of pyruvate by using a recombinant Escherichia coli YYC202 strain. This strain is completely blocked in its ability to convert pyruvate into acetyl-CoA or acetate, resulting in acetate auxotrophy during growth in glucose minimal medium. By controlling acetate and glucose feed rate, a series of lab-scale fed-batch experiments were performed at pH 7 and 37 °C. CO2 production rate (CTR) was used for on-line regulation of the acetate feed rate. The correlation between CTR and acetate consumption rate (ACR) was determined experimentally. At optimal process conditions a final pyruvate concentration higher than 62 g/L, a space-time yield of up to 42 g/L/d and pyruvate/glucose molar yield of 1.11 mol/mol were achieved. Experimental evidence was gathered that pyruvate export is active.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA