Naslov
The effect of n-3 polyunsaturated fatty acids (n-3 PUFAs) chicken eggs consumption on antioxidative enzymes gene exrpression in mononuclear blood cells in young healthy individuals

Autori
Šušnjara, Petar ; Mihaljević, Zrinka ; Matić, Anita ; Kolobarić, Nikolina ; Šafer, Iva ; Drenjančević, Ines

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
14thAnnual Meeting of Croatian Physiological Society with International Participation / - , 2020, 33-33

Skup
14thAnnual Meeting of Croatian Physiological Society with International Participation

Mjesto i datum
Virtual conference (online), 25.09.2020. - 26.09.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
antioxidant enzymes, gene expression, n-3 PUFA, vascular reactivity

Sažetak
Introduction: Oxidative stress underlies the mechanisms of impaired vascular reactivity associated with the development of cardiovascular disease. In previous studies (Appl Physiol Nutr Metab. 2018) we showed that there is a change in oxidative stress levels when consuming n-3 PUFA enriched eggs in healthy young subjects. Aim: The aim of the study was to determine the expression of the gene for SOD1-superoxy dismutase GPX1-glutathione peroxidase CAT- catalase MPO-myeloperoxidase Materials and methods: Gene expression of SOD1- superoxy dismutase (control group n = 7, experimental group n = 11), GPX1-glutathione peroxidase (control group n = 8, experimental group n = 9), CAT-catalase (control group n = 5, experimental group n = 6) and MPO- myeloperoxidase (control group n = 3, experimental group n = 5) in healthy young subjects who consumed n-3 PUFA-enriched eggs or plain, control eggs before and after the dietary protocol. Subjects consumed three boiled eggs daily for three weeks, the total amount of n-3 PUFA per day was 249 mg for the control group and 1053 mg n-3PUFA / day for the experimental group. Gene expression was determined by real-time PCR on a Bio Rad CFX96 device. Results: These preliminary results of gene expression of antioxidant enzymes SOD1, CAT, Gpx1 and MPO before and after consumption of n- 3 PUFA results do not show significant statistical differences. Conclusion: As our previous studies have shown the impact of n-3 PUFA-enriched eggs on oxidative stress levels, it is possible that changes at the gene level come later and more slowly. In order to determine which antioxidant enzymes mediate such a reduction, in addition to genetic expression, the protein expression of antioxidant enzymes should be determined and / or the activity of antioxidant enzymes should be determined. To draw concrete conclusions, the study should be repeated on a larger number of respondents.

Izvorni jezik
Engleski

POVEZANOST RADA