Naslov
Proizvodnja, pročišćavanje i karakterizacija enzima lipaza porijeklom iz Thermomyces lanuginosus
(Production, purification and characterization of lipase from Thermomyces lanuginosus)

Autori
Šibalić, Darijo ; Šalić, Anita ; Jurinjak Tušek, Ana ; Sokač, Tea ; Brekalo, Klara ; Zelić, Bruno ; Tišma, Marina

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts 18th Ružička days “TODAY SCIENCE – TOMORROW INDUSTRY” / Jukić, Ante - Zagreb : Osijek : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2020, 88-88

ISBN
978-953-6894-75-8

Skup
18. Ružičkini dani "Danas znanost - sutra industrija"

Mjesto i datum
Vukovar, Hrvatska, 16.09.2020. - 18.09.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Thermomyces lanuginosus ; lipase ; enzyme purification ; sustainability

Sažetak
The aim of the research was to determinate optimal process conditions for Thermomyces lanuginosus lipase (TLL) production during solid-state cultivation on hull-less pumpkin oil pomace. Numerical optimization of the five independent variables (substrate mass (ms), inoculum size (n), moisture content (wH2O), fermentation time (t) and temperature (T)) which affected the TLL production was evaluated using response surface methodology. To predict the TLL production, reduced quadratic mathematical model was proposed and model validation was performed under optimized conditions (ms = 50 g, n = 7, wH2O = 60%, t = 2 days, T = 45 °C) with 95% accuracy. The simplified purification method of the TLL was developed which includes a single-step vacuum-evaporation technique. Produced TLL liquid preparation with high volumetric activity (422 U cm-3) was obeyed. TLL was characterized as thermostable biocatalyst which possesses stability in a variety of organic solvents, food oils and fats. The sustainability of the process was confirmed by the green chemistry matrix of the E-factor and process mass index.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

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