Pregled bibliografske jedinice broj: 1080533
Inhibition of alfa-glucosidase by polyphenols present in traditional, indigenous apple varieties
Inhibition of alfa-glucosidase by polyphenols present in traditional, indigenous apple varieties // Book of abstracts 18th Ružička days “Today Science- Tomorrow Industry" / Jukić, Ante (ur.).
Zagreb : Osijek: Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2020. str. 100-100 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1080533 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Inhibition of alfa-glucosidase by polyphenols
present in traditional, indigenous apple varieties
Autori
Ištuk, Jozo ; Jakobek, Lidija ; Strelec, Ivica
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
Book of abstracts 18th Ružička days “Today Science- Tomorrow Industry"
/ Jukić, Ante - Zagreb : Osijek : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI) ; Prehrambeno tehnološki fakultet Sveučilišta Josipa Jurja Strossmayera u Osijeku, 2020, 100-100
ISBN
978-953-7005-72-6
Skup
18. Ružičkini dani "DANAS ZNANOST - SUTRA INDUSTRIJA"
Mjesto i datum
Vukovar, Hrvatska, 16.09.2020. - 18.09.2020
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
α-glucosidase ; inbition ; polyphenols ; apple
Sažetak
This work aimed to evaluate polyphenols from two traditional apple varieties as - glucosidase inhibitors. Polyphenols were extracted from the flesh and skin of two traditional apple varieties (Božićnica and Batulenka) by using ultrasonic- assisted extraction. Total polyphenols in extracts were determined by the spectrophotometric Folin- Ciocalteu method, expressed as gallic acid equivalents (GAE). The α-glucosidase activity was evaluated based on the spectrophotometric determination of p-nitrophenol (pNP) released from pnitrophenyl-α-D-glucopyranoside (p-NPG) substrate by the action of the enzyme. To inhibit enzyme activity, various polyphenol concentrations were added into the reaction mixture. IC50 value was calculated (concentration of polyphenols that gives 50% inhibition). Božićnica and Batulenka contained 337.24 and 202.17 mg GAE kg-1 in the flesh and 2132.91 and 824.43 mg GAE kg-1 in the peel, respectively. Polyphenols inhibited - glucosidase activity and inhibition reached a steady state. IC50 values, expressed as μg of gallic acid equivalents (GAE) per mL of reaction solution, of the flesh of Batulenka and Božićnica were 1.69 and 1.47, respectively. Peel showed similar IC50 values (1.07, and 0.33 μg GAE cm-3, for the peel of Batulenka and Božićnica, respectively). These results suggest that polyphenols from the flesh and skin of traditional apple varieties are potent inhibitors of α- glucosidase activity.
Izvorni jezik
Engleski
Znanstvena područja
Prehrambena tehnologija
POVEZANOST RADA
Projekti:
HRZZ-IP-2016-06-6777 - Utjecaj prehrambenih vlakana na bioraspoloživost polifenola istraživanjem adsorpcije i simuliranih probavnih procesa, in vitro (BIO-POLIFENOL) (Jakobek Barron, Lidija, HRZZ ) ( CroRIS)
Ustanove:
Prehrambeno-tehnološki fakultet, Osijek