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Pregled bibliografske jedinice broj: 1079889

Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry

Sarbu, Mirela; Raab, Shannon; Henderson, Lucas; Fabris, Dragana; Vukelić, Željka; Clemmer, David E.; Zamfir, Alina D.
Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry // Biochimie, 170 (2020), 36-48 doi:10.1016/j.biochi.2019.12.008 (međunarodna recenzija, članak, znanstveni)

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Naslov
Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry

Autori
Sarbu, Mirela ; Raab, Shannon ; Henderson, Lucas ; Fabris, Dragana ; Vukelić, Željka ; Clemmer, David E. ; Zamfir, Alina D.

Izvornik
Biochimie (0300-9084) 170 (2020); 36-48

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
collision-induced dissociation ; gangliosides ; human cerebrospinal fluid ; ion mobility separation/spectrometry mass spectrometry (IMS MS)

Sažetak
The proximity of cerebrospinal fluid (CSF) with the brain, its permanent renewal and better availability for research than tissue biopsies, as well as ganglioside (GG) shedding from brain to CSF, impelled lately the development of protocols for the characterization of these glycoconjugates and discovery of central nervous system biomarkers expressed in CSF. Currently, the investigation of CSF gangliosides is focused on concentration measurements of the predominant classes and much less on their profiling and structural analysis. Since we have demonstrated recently the high performance of ion mobility separation mass spectrometry (IMS MS) for compositional and structural determination of human brain GGs, in the present study we have implemented for the first time IMS MS for the exploration of human CSF gangliosidome, in order to generate the first robust mass spectral database of CSF gangliosides. IMS MS separation and screening revealed 113 distinct GG species in CSF, having similar compositions to the species detected in human brain. In comparison with the brain tissue, we have discovered in CSF several components containing fatty acids with odd number of carbon atoms and/or short glycan chains. By tandem MS (MS/MS) we have further analyzed the structure of GD3(d18:1/18:0) and GD2(d18:1/18:0), two glycoforms exhibiting short carbohydrate chains found in CSF, but discovered and characterized previously in brain as well. According to the present results, human CSF and brain show a similar ganglioside pattern, a finding that might be useful in clinical research focused on discovery of ganglioside species associated to neurodegenerative diseases and brain tumors.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)



POVEZANOST RADA

Projekti:
MZOS-108-1081870-2415 - Strukturno-funkcionalna glikolipidomika moždanog razvitka i maligne alteracije (Vukelić, Željka, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Željka Vukelić (autor)

Avatar Url Dragana Fabris (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Sarbu, Mirela; Raab, Shannon; Henderson, Lucas; Fabris, Dragana; Vukelić, Željka; Clemmer, David E.; Zamfir, Alina D.
Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry // Biochimie, 170 (2020), 36-48 doi:10.1016/j.biochi.2019.12.008 (međunarodna recenzija, članak, znanstveni)
Sarbu, M., Raab, S., Henderson, L., Fabris, D., Vukelić, Ž., Clemmer, D. & Zamfir, A. (2020) Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry. Biochimie, 170, 36-48 doi:10.1016/j.biochi.2019.12.008.
@article{article, author = {Sarbu, Mirela and Raab, Shannon and Henderson, Lucas and Fabris, Dragana and Vukeli\'{c}, \v{Z}eljka and Clemmer, David E. and Zamfir, Alina D.}, year = {2020}, pages = {36-48}, DOI = {10.1016/j.biochi.2019.12.008}, keywords = {collision-induced dissociation, gangliosides, human cerebrospinal fluid, ion mobility separation/spectrometry mass spectrometry (IMS MS)}, journal = {Biochimie}, doi = {10.1016/j.biochi.2019.12.008}, volume = {170}, issn = {0300-9084}, title = {Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry}, keyword = {collision-induced dissociation, gangliosides, human cerebrospinal fluid, ion mobility separation/spectrometry mass spectrometry (IMS MS)} }
@article{article, author = {Sarbu, Mirela and Raab, Shannon and Henderson, Lucas and Fabris, Dragana and Vukeli\'{c}, \v{Z}eljka and Clemmer, David E. and Zamfir, Alina D.}, year = {2020}, pages = {36-48}, DOI = {10.1016/j.biochi.2019.12.008}, keywords = {collision-induced dissociation, gangliosides, human cerebrospinal fluid, ion mobility separation/spectrometry mass spectrometry (IMS MS)}, journal = {Biochimie}, doi = {10.1016/j.biochi.2019.12.008}, volume = {170}, issn = {0300-9084}, title = {Cerebrospinal fluid: Profiling and fragmentation of gangliosides by ion mobility mass spectrometry}, keyword = {collision-induced dissociation, gangliosides, human cerebrospinal fluid, ion mobility separation/spectrometry mass spectrometry (IMS MS)} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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