Naslov
Cuticle-associated microbial communities of freshwater crayfish Astacus leptodactylus are affected by water temperature and the presence of pathogen Aphanomyces astaci

Autori
Vuk, Marija ; Orlić, Karla ; Burić, Lucija ; Maguire, Ivana ; Makkonen, Jenny ; Vladušić, Tomislav ; Šver, Lidija ; Hrašćan, Reno ; Hudina, Sandra ; Bielen, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts - 11th Symposium for European Freshwater Sciences-SEFS11 / - , 2019, 453-453

Skup
11th Symposium for European Freshwater Sciences (SEFS11)

Mjesto i datum
Zagreb, Hrvatska, 30.06.2019. - 05.07.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
no keywords

Sažetak
Native European crayfish species are in decline, mostly due to climate change-related habitat disturbances and spreading of oomycete pathogen Aphanomycesastaci. Increases in water temperature simultaneously affects the host and the pathogen, but also the host-associated microbial communities –an understudied component in host-pathogen relationship. Our aim was to characterize the cuticle-associated microbial communities of freshwater crayfish Astacus leptodactylusand to explore the potential changes in their composition related to water temperature and the presence of A. astaci. Crayfish were infected with A. astacizoospores at two water temperatures –control temperature (18 °C) and increased temperature as a simulation of global warming (22 °C). Next, we isolated the total DNA from cuticle swabs and analyzed the epibiontic microbiome composition by Illumina MiSeq 16S rDNA sequencing. Overall, microbialdiversity was significantly higher at 22 °C which could have influenced the increased host resistance to A. astaciobserved at this temperature (lower mortality). Some bacterial groups were predominantly present at lower temperature, such as Actinobacteria and Flavobacteriia, while members of Bacilli were more dominant at higher temperature. We also detected differences in microbial community composition regarding the presence of A. astaci. Ultimately, this is the first study that describes the microbial community shifts in freshwater crayfish related to climate change and A. astacipresence. As such, it should provide a starting point to determine the potential positive effects of epibiontic microbial communities on the host health.

Izvorni jezik
Engleski

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