Naslov
Monitoring of crayfish plague in the Plitvice lakes National park.

Autori
Pavić, Dora ; Bielen, Ana ; Hudina, Sandra ; Špoljarić, Ivana ; Grandjean, Frederic ; Jussila, Japo ; Maguire, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019) / - , 2019, 19-20

Skup
European International Association of Astacology meeting (IAA GOTLAND 2019)

Mjesto i datum
Gotland, Švedska, 27.08.2019. - 31.08.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Astacus astacus, Austropotamobius torrentium, Oomycetes, latent infection

Sažetak
Waterbodies of the Plitvice Lakes National Park are inhabited by two indigenous crayfish species, noble and stone crayfish. Native European crayfish populations are in decline, amongst others due to the lethal disease crayfish plague caused by pathogen Aphanomyces astaci. Presence of this pathogen was previously recorded in the Plitvice Lakes, but those analyses included small sample size. Thus, our goal was to analyse the presence of A. astaci on both crayfish species during two years monitoring period, using larger sample size, at multiple locations within the Park. For A. astaci detection we used IAA Gotland 201920non-invasive sampling method based on collecting swabs of mixed epibiotic microbial communities from crayfish carapace. DNA was isolated from swab samples and PCR was used to detect the presence of the pathogen. Positive samples were classified into semi-quantitative categories of pathogen load by qPCR, and microsatellite genotyping identified presence of strain A (As-genotype). Obtained results will be used in future management plans aiming to protect vulnerable native species in NP, with focus on prevention of unintentional spread of the pathogen.

Izvorni jezik
Engleski

POVEZANOST RADA