Naslov
Non-invasive detection of Aphanomyces astaci on individual crayfish

Autori
Pavić, Dora ; Čanković, Milan ; Sviličić Petrić, Ines ; Makkonen, Jenny ; Hudina, Sandra ; Maguire, Ivana ; Vladušić, Tomislav ; Šver, Lidija ; Hrašćan, Reno ; Orlić, Karla ; Dragičević, Paula ; Bielen, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Book of abstracts - European International Association of Astacology meeting (IAA GOTLAND 2019) / - , 2019, 20-20

Skup
European International Association of Astacology meeting (IAA GOTLAND 2019)

Mjesto i datum
Gotland, Švedska, 27.08.2019. - 31.08.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
pathogenic oomycete, native crayfish species, biofilm

Sažetak
Pathogenic oomycete Aphanomyces astaci is transmitted mainly by invasive North American crayfish, and causes the crayfish plague, a disease mostly lethal for native European crayfish. Detecting the pathogen in endangered native crayfish populations before the disease outbreak would provide a starting point in the development of effective control measures. However, current A. astaci-detection protocols either rely on eDNA isolated from large volumes of water or, if focused on individual animals, include crayfish killing. Thus, we have developed a non-invasive method that detects A. astaci DNA in microbial biofilm associated with the cuticle of individual crayfish, bypassing the unnecessary killing step. Efficiency of the new method was confirmed by PCR and qPCR and the obtained results were congruent with the traditional invasive method. We propose that the new method should be used in future monitoring of A. astaci presence in endangered European native crayfish individuals.

Izvorni jezik
Engleski

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