Naslov
Naphthalene diimide–amino acid conjugates as novel fluorimetric and CD probes for differentiation between ds-DNA and ds-RNA

Autori
Weißenstein, Annike ; Vysotsky, Myroslav O. ; Piantanida, Ivo ; Würthner, Frank

Izvornik
Beilstein journal of organic chemistry (2195-951X) 16 (2020); 2032-2045

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
amino acid–fluorophore conjugate ; circular dichroism ; DNA/RNA recognition ; fluorescence ; intercalation ; naphthalene diimide

Sažetak
Two novel unnatural amino acids, prepared by linking a dicationic purple colored and fluorescent naphthalene diimide (NDI) at core position to amino acid side chains of variable length, strongly interacted with ds-DNA/RNA by threading intercalation. Different from a reference NDI dye with identical visible range absorbance (520-540 nm) and Stokes shifts in emission (+60 nm, quantum yield > 0.2), only these amino acid-NDI conjugates showed selective fluorimetric response for GC-DNA in respect to AT(U)- polynucleotides. The DNA/RNA binding-induced circular dichroism (ICD) response of NDI at 450-550 nm strongly depended on the length and rigidity of the linker to the amino acid unit, which controls the orientation of the NDI unit inside within the intercalative binding site. The ICD selectivity also depends on the type of polynucleotide, thus studied NDI dyes act as dual fluorimetric/ICD probes for sensing the difference between here used GC-DNA, AT-DNA and AU-RNA

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA