Naslov
Airborne fungi in dwelings and grain mill in Croatia: a year round investigation

Autori
Jakšić Despot, Daniela ; Šegvić Klarić, Maja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Power of microbes in industry and environment, Book of Abstracts / Teparić, Renata ; Frece, Jadranka ; Mrša, Vladimir - Zagreb : Hrvatsko mikrobiološko društvo, 2013, 42-42

ISBN
978-953-7778-06-4

Skup
Power of microbes in industry and environment

Mjesto i datum
Primošten, Hrvatska, 09.10.2013. - 12.10.2013

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Recenziran

Ključne riječi
Airborne fungi, Aspergillus, grain mill, apartment, basement

Sažetak
The aim of this study was to assess qualitative and quantitative composition of aeromycota based on airsampling conducted during one year in two-month intervals in Zagreb, Croatia at the locations including grain mill (GM), apartment (AP) and basement (BS) compared to the outdoor air (ODA). In addition, the incidence of potentially harmful species of Aspergilli belonging to the sections Flavi, Nigri and Versicolores has been observed. The number of fungal spores (CFU/m3) was scored based on colony growth after five days of incubation on DG-18 agar plates (Dichloran-18% glycerol-agar) at 25 °C for each sample (420 in total). Isolation and determination of the fungi based on morphological characteristics on the appropriate culture media like CYA (Czapek Yeast Extract Agar) followed. The average concentrations of airborne fungi scored in grain mill samples were significantly higher (P<0.0001) during whole year compared to all other sampling sites and in range from 14310- 40000 CFU/m3. Statistically estimated values were up to six times higher compared to measured values, therefore potentially dangerous for the health of occupationally exposed workers. Measured and statistically estimated concentrations of airborne fungi scored in BS were statistically higher than those in AP in January (P=0.0008), March, September and November (P<0.0001). In November, concentrations of airborne fungi scored in BS were statistically higher than in ODA. Interestingly, concentrations scored in ODA were statistically higher than those in AP in January (P=0.0008), March, July and September (P<0.0001). The concentrations of airborne fungi in ODA varied from 286 in January to 2090 CFU/m3 in June. The most abundant species throughout the year were Cladosporium spp. (90- 100%), Penicillium spp. (40-100%) and Alternaria spp. (10-100 %), which is common in temperate climates. Aspergillus spp. and their teleomorph Eurotium spp. (85-100%) were the most abundant species in the grain mill while the species belonging to the section Flavi (50- 100%), section Nigri ( 15-40%) and A. ochraceus ( 15-60%) were the most prevalent. In addition, these species were rarely found in outdoor air and therefore location specific. In dwellings, the average concentrationof fungi ranged from 163-1244 CFU/m3 in AP and in BS from 697-1123 CFU/m3. At these sites Aspergillus spp. were more abundant than in ambient air in the period from March to September with domination of section Versicolores. In conclusion, there is an overload with aeromycoparticles in the grain mill throughout a year. Concentartions scored in AP were always lower than in ODA, and lower than in BS except in warmer period of year when they were quite similar. In addition, grain mill is supportive location for species belonging to the section Flavi, while species from the section Versicolores are more common in dwellings. Aspergilli belonging to the section Nigri were found at all locations, but they were more prevalent in grain mill than at dwellings.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Interdisciplinarne prirodne znanosti, Javno zdravstvo i zdravstvena zaštita, Farmacija

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