Naslov
Experimental Investigation and Modelling of Bacterial Growth and Inactivation: E. coli Exposed to Laser-synthesized Silver Nanoparticles

Autori
Krce, Lucija

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Prirodoslovno-matematički fakultet

Mjesto
Split

Datum
03.07

Godina
2020

Stranica
97

Mentor
Aviani, Ivica

Ključne riječi
bacterial growth, E. coli, growth rate, logistic model, silver nanoparticles, antibacterial activity, modelling bacterial growth, laser-synthesized nanoparticles, mode of antibacterial action, nano-bio interactions, reactive oxygen species, atomic force microscopy, Young modulus.

Sažetak
The aim of this thesis is to elaborate on the mode of antibacterial action by colloidal silver nanoparticles against Escherichia coli as a model organism. Two approaches were used to investigate the bactericidal mechanism of silver nanoparticles (AgNPs): experimental probing and modelling of the treated bacterial cell growth. AgNPs were synthesized by laser ablation in water and the obtained colloid was thoroughly characterized. An upgradable interactionbased growth model was developed to understand the effect of AgNPs on bacterial cell dynamics. The model explains untreated and AgNP- treated Escherichia coli growth in batch culture. Subsequently, fluorescence probing and atomic force microscopy and spectroscopy were used to experimentally study the AgNP bactericidal mechanism. This study shows that the mode of antibacterial action of silver nanoparticles includes penetration of AgNPs inside the cell/membrane, membrane permeabilization, reduction of the cell’s Young modulus and possible cell leakage while undistinguished reactive oxygen species level was detected inside lethally treated cells.

Izvorni jezik
Engleski

Znanstvena područja
Fizika, Interdisciplinarne prirodne znanosti

POVEZANOST RADA