Naslov
Isolation, solubilization and biological activity of isoflavonoids from Fabaceae family

Autori
Fumić, Barbara

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, doktorska disertacija

Fakultet
Farmaceutsko-biokemijski fakultet

Mjesto
Zagreb

Datum
04.06

Godina
2020

Stranica
109

Mentor
Zovko Končić, Marijana ; Jug, Mario

Ključne riječi
Cyclodextrines ; isoflavones ; Lotus corniculatus ; Medicago sativa ; mucopolysaccharidoses

Sažetak
Mucopolysaccharidoses are hereditary metabolic diseases characterized by the accumulation of various glycosaminoglycans in the tissues of the diseased. Isoflavones are secondary plant metabolites that can slow down the synthesis of glycosaminoglycans, but their therapeutic potential is significantly limited by their poor oral bioavailability. In this thesis, the ethanol and cyclodextrin ultrasound-assisted extraction of aerial parts of two plant species from the family Fabaceae, Medicago sativa and Lotus corniculatus, was compared. The study was conducted using response surface methodology based on a Box- Benkhen design with three independent variables. The influence of isoflavones, the prepared extracts and inclusion complexes of selected isoflavones with different cyclodextrins on oxidative stress and glycosaminoglycan synthesis in fibroblast cell lines of patients with mucopolysaccharidosis was evaluated. Although ethanol was a more suitable solvent for the extraction of total polyphenols from M. sativa, the use of hydroxypropyl--cyclodextrin allowed for a more selective extraction of total flavonoids whose amount was as much as three times higher than in ethanol extracts. The amount of total flavonoids extracted using cyclodextrin from L. corniculatus was two times greater than the amount of total flavonoids of the species M. sativa. The cyclodextrin complexes of these extracts showed, depending on the dose, a significant decrease in glycosaminoglycan levels in fibroblast cells of patients with mucopolysaccharidoses, with no significant cytotoxic effect.

Izvorni jezik
Engleski

Znanstvena područja
Farmacija

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