Naslov
Flexibility and Preorganization of Fluorescent Nucleobase-Pyrene Conjugates Control DNA and RNA Recognition

Autori
Ban, Željka ; Matić, Josipa ; Žinić, Biserka ; Foller Füchtbauer, Anders ; Wilhelmsson, L. Marcus ; Piantanida, Ivo

Izvornik
Molecules (1420-3049) 25 (2020), 9; 2188, 19

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
DNA/RNA recognition ; hydrogen bonding ; fluorescent nucleobase ; pyrene ; intramolecular pre-organization

Sažetak
We synthesized a new amino acid-fluorescent nucleobase derivative (qAN1-AA) and from it two new fluorescent nucleobase–fluorophore (pyrene) conjugates, whereby only the analogue with the longer and more flexible linker (qAN1-pyr2) self-folded into intramolecularly stacked qAN1/pyrene conformation, yielding characteristic, 100 nm-red-shifted emission (λmax = 500 nm). On the contrary, the shorter and more rigid linker resulted in non-stacked conformation (qAN1- pyr1), characterized by the emission of free pyrene at λmax = 400 nm. Both fluorescent nucleobase– fluorophore (pyrene) conjugates strongly interacted with ds-DNA/RNA grooves with similar affinity but opposite fluorescence response (due to pre-organization), whereas the amino acidfluorescent base derivative (qAN1-AA) was inactive. However, only intramolecularly self-folded qAN1-pyr2 showed strong fluorescence selectivity toward poly U (Watson–Crick complementary to qAN1 nucleobase) and poly A (reverse Hoogsteen complementary to qAN1 nucleobase), while an opposite emission change was observed for non-complementary poly G and poly C. Non-folded analogue (qAN1-pyr1) showed no ss-RNA selectivity, demonstrating the importance of nucleobasefluorophore pre-organization.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA