Naslov
Chemical Reactions in Phospholipid Bilayers and Their Relevance to Membrane Biophysics

Autori
Škulj, Sanja ; Vazdar, Katarina ; Vazdar, Mario

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
In­ter­na­tional Work­shop on Biological Mem­branes

Mjesto i datum
Helsinki, Finska, 19.08.2018. - 22.08.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
oxidative stress ; 4-HNE ; PE lipids

Sažetak
In conditions of oxidative stress, different reactive oxidative species (ROS) are constantly produced in living cells. Among the plethora of changes in biophysical membrane properties, ROS are also altering ion transport across phospholipid bilayers resulting in the disorder in cell metabolism. The most prominent ROS formed in cellular membranes during oxidative stress is aldehyde 4-hydroxy-2E-nonenal (4-HNE), which readily reacts with surrounding membrane proteins and lipids containing amino groups. In order to shed more light on the consequences of chemical changes on membrane biophysical properties, we modified amino groups of phosphoethanolamine (PE) to match experimentally observed covalent PE adducts [3, 4]. We performed molecular dynamics (MD) simulations of bilayers with different lipid compositions supported with mechanistic details of chemical reactions in membranes described with highly detailed quantum (QM) chemical calculations and different experimental techniques in model systems, such as nuclear magnetic resonance (NMR) and liquid chromatography tandem mass spectrometry (LC-MS). Using this synergistic approach, we describe how specific chemical reactions of 4-HNE with PE lipids resulting in complex PE adducts induce a drastic increase of Na+ transport across model biological membranes, in contrast to other simpler PE adducts which do not significantly alter membrane biophysical properties.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA