Naslov
Pentadecapeptide BPC 157 Counteracts Thrombosis and All Manifestations of 48 h-Inferior Cava Vein Ligation (Syndrome) in Rats

Autori
Vukojević, Jakša ; Siroglavić, Marko ; Kašnik, Katarina ; Kralj, Tamara ; Stanćić, Duje ; Kokot, Antonio ; Kolarić, Darko ; Drmić, Domagoj ; Zenko Sever, Anita ; Barišić, Ivan ; Šuran, Jelena ; Bojić, Davor ; Patrlj Hrelec, Maša ; Sjekavica, Ivica ; Brčić, Luka ; Stupnišek, Mirjana ; Seiwerth, Sven ; Sikirić, Predrag

Izvornik
Basic & clinical pharmacology & toxicology (1742-7835) 115 (2014), Suppl. 1; 40-40

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Pentadecapeptide ; BPC 157 ; Thrombosis ; Rats

Sažetak
Background: We implicated stable gastric pentadecapeptide BPC 157 particular beneficial combining (bleeding/thrombocytopenia after amputation and/or anticoagulants and aspirin and abdominal aorta anastomosis-thrombosis both counteracted ; wound healing capacity ; endothelium protection ; interactions with NO- system in different models and various species ; particular effect on blood pressure ; antiarrhythmic effect) to counteract deep vein thrombosis (DVT) and inferior caval vein (ICV)- syndrome. Methods: Surgery. In deeply anaesthetized female Wistar rats the ICV was exposed via a midline laparotomy, dissected at the level of the right ovarian vein, and then ligated up to right ovarian vein, and rats were randomly assigned in different groups for further experiments and assessments (ICV and aorta blood pressure recordings, ECG recordings, heart rate, blood vessel collateralization, bleeding, vein wall analysis, termography, indirect ophtalmoscopy of retina). BPC 157 was given as 1 ml bath (thrombosis ; blood pressure ; heart rate ; collaterization) ; or intraperitoneally (bleeding). Delayed therapy. BPC 157 (10 lg/kg, 10 ng/kg) was given intraperitoneally, at advanced ligation-time points: 1 h ; 2 h ; 6 h, 24 h, 48 h. Controls simultaneously received an equivolume of saline (5 ml/kg). Assessments were carried out at 10 min, 30 min, 1 h, 6 h, 24 h and 48 h thereafter. Results: In rats with 48 h-ICV ligation, BPC 157 salutary effect was elicited at any point of 48 h- ligation-time. BPC 157 (10 lg, 10 ng/kg, both early (i.e. 1 ml bath at 5 min ligation-time) and delayed therapy (intraperitoneally)) counteracts thrombosis and all manifestations of 48 h-ICV ligation (syndrome), i.e. instead progressive DVT, the length and weight of the formed clot considerably less ; instead thickened, intima as thick as the nuclei of normal spindle-shaped endothelial cells ; instead substantially increased bleeding with injury and thrombocytopenia, BPC 157-rats bleed less, and have no thrombocytopenia ; blood pressure brought to normal values in both ICV and abdominal aorta, vein hypertension and systemic hypotension counteracted, almost normal eye fundus, tachycardia normalized, attenuated ischemia, gross organ congestion greatly prevented or reduced, collaterals and redistribution of otherwise trapped blood volume rapidly presented, and thereby attenuated drinking, leg edema and cyanosis. Conclusions: Pentadecapeptide BPC 157 counteracts DVT and all manifestations of 48 h-ICV ligation (syndrome) in rats. This considers its effect on endothelium and vessels and bleeding, wound healing ; NO-system ; blood pressure ; anti- arrhythmic effect.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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