Naslov
Analysis of multiple loci can increase reliability of detection of fetal Y-chromosome DNA in maternal plasma

Autori
Wagner, Jasenka ; Džijan, Snježana ; Pavan-Jukić, Doroteja ; Wagner, Jadranka ; Lauc, Gordan

Izvornik
Prenatal Diagnosis (0197-3851) 28 (2008), 5; 412-416

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Cell-free fetal DNAPolymorphic STR lociPrenatal gender determinationY-chromosome DNA

Sažetak
Objective Aiming to develop more reliable methods for determination of fetal gender from maternal plasma we compared three different systems of polymerase chain reaction (PCR) detection of Y- chromosome DNA. Methods Cell- free DNA was isolated from 96 samples of maternal plasma and (1) amplified using AmpFLSTR-Identifiler (15 autosomal STR loci and amelogenin) or AmpFLSTR- Yfiler (16 Y-chromosome STR loci) kits and subsequently analyzed on ABI-PRISM 310 Genetic Analyzer, or (2) analyzed using Quantifiler-Y DNA-Quantification kit. Gender of fetuses was confirmed by cytogenetic analysis or pheno- typically at birth. Results and Conclusions AmpFLSTR-Identifiler and Quantifiler-Y Human- Quantification kits were rather reliable in determining fetal gender (92.5 and 98.1%, respectively), but false negatives were still present in both systems. AmpFLSTR-Yfiler was found to be fully reliable as it amplified Y- chromosome in all cases of male fetuses, and was thus 100% correct in determining fetal gender. In addition, it enabled comparison of polymorphic Y- chromosome loci between father and a child, thus further supporting specificity of obtained results. Copyright © 2008 John Wiley & Sons, Ltd.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA