Naslov
A comparison of activating agens for efficiency and stability of aldolase immobilized on magnetic nanoparticles

Autori
Skendrović, Dino ; Rimac, Tomislav ; Vrsalović Presečki, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka XIII. SMLKI 2020 / - , 2020, 77-77

Skup
XIII. skup mladih kemijskih inženjera

Mjesto i datum
Zagreb, Hrvatska, 20.02.2020. - 21.02.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Nije recenziran

Ključne riječi
Aldolase, immobilization, magnetic nanoparticles

Sažetak
Enzyme immobilization is a promising technique for improving enzyme stability while retaining its activity. It often includes binding of an enzyme to a solid carrier, by employing covalent bonds or by adsorption. If using covalent bond, the carriers should be activated with appropriate activating agents that act as a link between carrier and enzyme. The aim of this work was to immobilize aldolase on silica coated magnetic nanoparticles. For that purpose carrier was synthesized using solvothermal method and three different types of activating agents, glutaraldehyde, benzoquinone and 3-Glycidyloxypropyl trimethoxysilane (GPTMS), were used (Figure 1). The features of immobilized aldolase were examined in the reaction of double aldol addition for production of statin side chain precursors, a valuable pharmaceutical intermediate. Retained activity and enzyme stability of immobilized aldolase was compared with that of a free enzyme.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA