Naslov
An outbreak of gastroenteritis due to Aeromonas hydrophila

Autori
Carev, Merica ; Tandara, Danica ; Rizvan Pero ; Barišić, Zvonimir ; Šisko Kraljević, Katarina ; Borzić, Elmica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Clinical Microbiology and Infection / - Kopenhagen : ESCMID, 2005, 420-420

Skup
15th European Congress of Clinical Microbiology and Infection

Mjesto i datum
Kopenhagen, Danska, 02.04.2005. - 05.04.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
gastroenteritis ; Aeromonas hydrophila

Sažetak
Objectives: Our goal was to report an outbreak of Aeromonas hydrophila gastrointestinal infection due to consumption of contaminated water, in the rural area of Dalmatia County, Croatia. This report warns about diagnostic difficulties and importance of finding Aeromonas in both water and human stool samples. Methods: Our epidemiologist personally interviewed 30 symptomatic and 36 asymptomatic villagers. Four human stools (13.3%) from the diseased were analysed on: Salmonella spp., Shigella spp., Yersinia spp., Vibrio spp., Campylobacter spp., Rota and Adenoviruses, intestinal protozoa and helmint eggs. Six water samples were examined bacteriologically, virologically and parasitologically. Results: All diseased were drinking water from local drinking water distribution system, with pipelines very distant from the water source. Main symptoms were abdominal pain, watery diarrhoea and tiredness. Most of the patients recovered completely after 48 hrs and were only treated symptomatically. 28 persons (78%) from control group didn’t drink that water at all. All stool samples were negative on viruses, parasites and bacterial pathogens. The result of water analyses was negative for viruses and intestinal parasites. Bacteriological analyses of first water sample showed that the number of aerobic mesophilic and psychrophilic bacteria per ml was higher than standard. A. hydrophila was isolated from Cefsulodin Irgasan Novobiocin (CIN) agar (>1800 CFU/100 ml). Further analysis of the last two stool samples included additional testing of pink colonies from CIN agar. They were transferred on Kligler Iron Agar and incubated at 37ºC and 25ºC. Oxydase and catalase tests were positive and Api 20E and BBL crystals confirmed isolates as A. hydrophila. Stool and water isolates showed same susceptibility patterns for antibiotics (resistance to amoxicillin and cefazolin). This was the first confirmed isolation of these bacteria as cause of an outbreak in our County. After cleaning, chlorination and connection to new water source, water samples were in accordance to the standards. There were no new cases. Conclusion: Identical biochemical and antimicrobial susceptibility patterns of human and water strains, with positive clinical findings and epidemiological data showed us that this small outbreak was due to A. hydrophila. This conclusion was supported by negative tests for other pathogens. Real prevalence of this pathogen is still unknown and probably underestimated.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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