Naslov
Tuberculotic orchiepididimytis – A case report

Autori
Barišić, Zvonimir ; Vrsalović Nenad ; Milostić Kazimir ; Alfirević Darko ; Babić-Erceg Andrea ; Borzić, Elmica ; Zoranić, Vinko ; Kaliterna, Vanja ; Carev, Merica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, ostalo

Izvornik
Abstract book of 23rd Annual Congress of the European Society of Mycobacteriology / Katalinić - Janković, Vera - Zagreb : European Society of Mycobacteriology, 2002, 99-99

Skup
23rd Annual Congress of the European Society of Mycobacteriology

Mjesto i datum
Dubrovnik, Hrvatska, 23.06.2002. - 26.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Tbc ; molekularna dijagnostika
(tuberculosis ; molecular diagnostics)

Sažetak
A 39-year-old male was admitted by urologist for the first time in May 2000 because of swallowing of left testicle and epididymis. He suffered from pulmonary sarcoidosis eight years before. Testicle changes evoluted from swalowing to fistula formation on left scrotum with suppurative discharge. He was treated with ciprofloxacin that did not produce any improvement of disease, so that in March 2001 surgical extirpation of intlamatory destroyed left testicle and epidydimis was performed. Bacteriological analysis of testicle tissue showed presence of Proteus mirabilis and beta haemolytic streptococcus, while acid-fast microscopy as well as culture did not show presence of tubercle bacilli. Microscopically the structure of testis and epididymis was almost completely obliterated by numerous tubercles which became confluent to produce large caseous masses with sourounding heavy inflamatory mononuclear infiltrate of plasma cells and lymphocites with numerous giant multinucleate Langhans cells, suggesting tuberculous ethiology. After that, exudate from surgical wound was examined on presence of Mycobacterium tuberculosis DNA by nucleic acid amplification method ligase chain reaction (LCx Mycobacterium tuberculosis Assay, Abbott Laboratories, USA). Positive assay clearly proved ethiology of orchiepididymitis, and after that patient was treated with antituberculotics, which completely cured inflamatory process. Nucleic acid amplification methods, where ligase chain reaction also belongs to, have high specificity for detecting target sequence of DNA and result can be obtained in one day. These techniques are useful in diagnostics of tuberculosis, especially in cases when small amount of tubercle bacilli was present. In our patient acid-fast microscopy as well as culture did not show ethiological agents, but microscopical examination of tissue and nucleic acid amplification method ligase chain reaction confirmed that cause of orhiepididymitis was M. tuberculosis. Mycobacteriological diagnostics in Public Health Institute of Split have a long tradition and it started by foundation of Bacteriological Station in 1922. In the beginning it was only acid-fast microscopy. After 1933, when Bacteriological Station developed into Institute for Hygiene, it started with cultivation of M. tuberculosis. Nucleic acid amplification method for diagnostics of M. tuberculosis is performed since 2001.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti, Javno zdravstvo i zdravstvena zaštita

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