Naslov
Bacillus spp. in biological control of cultivated mushroom diseases

Autori
Svetlana Milijašević-Marčić, Olja Stanojević, Emil Rekanović, Gabriella Kanižai Šarić, Ankica Sarajlić, Ivana Majić, Ivana Potočnik

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Zbornik sažetaka 55. hrvatskog i 15. međunarodnog simpozija agronoma / Boro Mioč, Ivan Šarić - Zagreb : Agronomski fakultet Sveučilišta u Zagrebu, 2020, 41-41

Skup
55. hrvatski i 15. međunarodni simpozij agronoma

Mjesto i datum
Vodice, Hrvatska, 16.02.2020. - 21.02.2020

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mushrooms ; green mould disease ; Bacillus spp. ; antagonistic activity ; biocontrol

Sažetak
The objective of the study was to obtain potential biocontrol agents of green mould disease, caused by Trichoderma spp., affecting the most commonly cultivated basidiomycetes: white button mushroom (Agaricus bisporus) and oyster mushroom (Pleurotus spp.). The isolation of bacterial strains with antagonistic properties against Trichoderma spp. was carried out from samples of straw and chicken manure, compost at various stages of the composting process and casing soil used for growing button mushrooms. Bacterial isolates characterized as Gram-positive and catalase- positive were identified based on the hypervariant region of the 16S rDNA as: Bacillus subtilis, B. amyloliquefaciens, B. licheniformis and B. pumilus. After a preliminary screening of bacterial isolates for antagonistic activity against Trichoderma aggressivum f. europaeum further screening was conducted with additional indicator isolates of fungi: T. harzianum, T. koningii and T. atroviride. Growth inhibition of Trichoderma spp. ranged from 40 to 70 % depending on the antagonistic strain and fungal indicator. The strains with the strongest antagonistic activity were selected for in vivo experiments in which the efficacy of strains in pathogen suppression and their effect on mushroom yield were evaluated, in comparison with a commercial biofungicide based on Bacillus velezensis QST 713 and a fungicide based on prochloraz-Mn. In mushroom growing room trials against T. aggressivum f. europaeum, the most effective treatment in pathogen control was prochloraz-Mn, followed by the native B. amyloliquefaciens B-241, B. velezensis QST 713, B. pumilus B-138 and B. subtilis B- 233. Strain B. amyloliquefaciens B- 241 showed the greatest potential for biocontrol of green mould disease in button mushroom production caused by various Trichoderma species, which makes it a good candidate for further trials at commercial scale. In addition, antagonistic potential of ten chosen Bacillus subtilis strains was evaluated against causal agents of green mould disease of oyster mushroom, Trichoderma pleuroti and Trichoderma pleuroticola. Based on the in vitro method for quantification of antagonistic potential of Bacillus spp. strains, growth inhibition of T. pleuroti ranged from 55 to 62 % and no significant differences among antagonistic activity of tested B. subtilis strains were found. Inhibition of T. pleuroticola was slightly higher, ranging from 55 to 70 % and strain B. subtilis B- 358 induced the highest growth inhibition thus showing promising results for further in vivo trials. This research confirmed mushroom substrate to be a good source of antagonistic microorganisms with potentials for use in biological control of green mould control in edible mushroom production. This work was supported by bilateral project 337- 00- 205/2019-09/21.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija)

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