Pregled bibliografske jedinice broj: 1049729
Cytomegalovirus Infection: Mouse Model
Cytomegalovirus Infection: Mouse Model // Current Protocols in Immunology, 122 (2018), 1; 1-23 doi:10.1002/cpim.51 (međunarodna recenzija, članak, znanstveni)
CROSBI ID: 1049729 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
Cytomegalovirus Infection: Mouse Model
Autori
Brizić, Ilija ; Lisnić, Berislav ; Brune, Wolfram ; Hengel, Hartmut ; Jonjić, Stipan
Izvornik
Current Protocols in Immunology (1934-3671) 122
(2018), 1;
1-23
Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni
Ključne riječi
mice ; cytomegalovirus infections/virology ; disease models ; animal ; virus replication ; viral plaque assay/methods ; viral load ; salivary gland/virology ; mouse cytomegalovirus/isolation & purification ; herpesviridae infections/virology ; fibroblasts/virology ; MCMV
Sažetak
This unit describes procedures for infecting newborn and adult mice with murine cytomegalovirus (MCMV). Methods are included for propagating MCMV in cell cultures and for preparing a more virulent form of MCMV from salivary glands of infected mice. A plaque assay is provided for determining MCMV titers of infected tissues or virus stocks. Also, a method is described for preparing the murine embryonic fibroblasts used for propagating MCMV and for the plaque assay.
Izvorni jezik
Engleski
Znanstvena područja
Temeljne medicinske znanosti
POVEZANOST RADA
Ustanove:
Medicinski fakultet, Rijeka
Citiraj ovu publikaciju:
Časopis indeksira:
- Scopus
- MEDLINE
